Product nameAnti-SDHA antibody [2E3GC12FB2AE2] (Alexa Fluor® 594)
See all SDHA primary antibodies
DescriptionMouse monoclonal [2E3GC12FB2AE2] to SDHA (Alexa Fluor® 594)
ConjugationAlexa Fluor® 594. Ex: 590nm, Em: 617nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Cow, Dog, Human
Tissue, cells or virus corresponding to Cow SDHA. Purified mitochondrial complex II (Cow).
Database link: P31039
- HeLa and HDFn cells.
Product was previously marketed under the MitoSciences sub-brand.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab170172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab178000 - Mouse monoclonal IgG1 (Alexa Fluor® 594), is suitable for use as an isotype control with this antibody.
FunctionFlavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Involvement in diseaseDefects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CMD1GG antibody
- DHSA_HUMAN antibody
- Flavoprotein subunit of complex II antibody
ab170172 staining SDHA in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab170172 at a 1/1000 dilution (shown in pseudo color red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
Immunocytochemistry with HDFn (100x) cells.
A) HDFn stained with anti-SDHA Alexa-594 antibody (1.0 µg/mL). B) HDFn stained with Anti-mitochondrial chaperone HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.
Immunocytochemistry with HeLa cells (100x).
A) HeLa stained with anti-SDHA Alexa-594 antibody (1.0 µg/mL). B) HeLa stained with Anti-HSP60 (1/1000, ab46798), Secondary antibody used was goat anti-rabbit Alexa® 488 (1/1000, ab150077). C) DAPI as nuclear stain (1/10000). D) Merge of color channels to show specificity of signal to mitochondria.
ab170172 has not yet been referenced specifically in any publications.