Product nameAnti-SDHA antibody
See all SDHA primary antibodies
DescriptionRabbit polyclonal to SDHA
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Cow, Pig, Cynomolgus monkey, Orangutan
Recombinant full length protein corresponding to Human SDHA aa 44-664.
SAKVSDSISAQYPVVDHEFDAVVVGAGGAGLRAAFGLSEAGFNTACVTKL FPTRSHTVAAQGGINAALGNMEEDNWRWHFYDTVKGSDWLGDQDAIHYMT EQAPAAVVELENYGMPFSRTEDGKIYQRAFGGQSLKFGKGGQAHRCCCVA DRTGHSLLHTLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIEDGS IHRIRAKNTVVATGGYGRTYFSCTSAHTSTGDGTAMITRAGLPCQDLEFV QFHPTGIYGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVV SRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGV DVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAAC ASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESV MNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLY GDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHA REDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRP VIDKTLNEADCATVPPAIRSY
Database link: P31040
- WB: HeLa whole cell lysate; Mouse liver and spleen lysates; Rat heart lysate; Zebrafish lysate. IHC-P: Human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab235594 in the following tested applications.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 72 kDa.|
FunctionFlavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
PathwayCarbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
Involvement in diseaseDefects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similaritiesBelongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CMD1GG antibody
- DHSA_HUMAN antibody
- Flavoprotein subunit of complex II antibody
All lanes : Anti-SDHA antibody (ab235594) at 1/500 dilution
Lane 1 : Rat heart lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse liver lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : Zebrafish lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 72 kDa
Paraffin-embedded human kidney tissue stained for SDHA using ab235594 at 1/50 dilution in immunohistochemical analysis.
ab235594 has not yet been referenced specifically in any publications.