Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SDHA antibody [EPR9042(B)] (ab139181)

Rabbit recombinant monoclonal SDHA antibody [EPR9042(B)]. Validated in WB, IP, IHC, Flow Cyt and tested in Mouse, Rat, Human.

Overview

  • Product name

    Anti-SDHA antibody [EPR9042(B)]
    See all SDHA primary antibodies
  • Description

    Rabbit monoclonal [EPR9042(B)] to SDHA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SDHA aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, fetal kidney, HepG2 and HeLa lysate; Human kidney and testis tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab139181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function

      Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
    • Pathway

      Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
    • Involvement in disease

      Defects in SDHA are a cause of mitochondrial complex II deficiency (MT-C2D) [MIM:252011]. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations. Clinical features include psychomotor regression in infants, poor growth with lack of speech development, severe spastic quadriplegia, dystonia, progressive leukoencephalopathy, muscle weakness, exercise intolerance, cardiomyopathy. Some patients manifest Leigh syndrome or Kearns-Sayre syndrome.
      Defects in SDHA are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
      Defects in SDHA are the cause of cardiomyopathy dilated type 1GG (CMD1GG) [MIM:613642]. CMD1GG is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities

      Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
    • Cellular localization

      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CMD1GG antibody
      • DHSA_HUMAN antibody
      • Flavoprotein subunit of complex II antibody
      • Fp antibody
      • PGL5 antibody
      • SDH 2 antibody
      • SDH1 antibody
      • SDH2 antibody
      • SDHA antibody
      • SDHF antibody
      • Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody
      • Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody
      • Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibody
      • Succinate dehydrogenase complex flavoprotein subunit A antibody
      • Succinate dehydrogenase complex flavoprotein subunit antibody
      • Succinate dehydrogenase complex flavoprotein subunit precursor antibody
      • Succinate dehydrogenase complex subunit A antibody
      • Succinate dehydrogenase complex subunit A flavoprotein (Fp) antibody
      • Succinate dehydrogenase complex subunit A flavoprotein antibody
      see all

    Images

    • All lanes : Anti-SDHA antibody [EPR9042(B)] (ab139181) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293 whole cell lysate
      Lane 2 : SDHA knockout HEK-293 whole cell lysate
      Lane 3 : MCF7 whole cell lysate
      Lane 4 : HepG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 72 kDa
      Observed band size: 72 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab139181 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab139181 was shown to recognize SDHA in wild-type HEK-293 cells as signal was lost at the expected MW in SDHA knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHA knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab139181 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-SDHA antibody [EPR9042(B)] (ab139181) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 72 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling SDHA with ab139181 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling SDHA with ab139181 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab139181 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab139181 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Malty RH  et al. A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-?B Signaling. Cell Syst 5:564-577.e12 (2017). Read more (PubMed: 29128334) »
    See 1 Publication for this product

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