Anti-SDHAF1 antibody - C-terminal (ab219764)

Overview

  • Product name

    Anti-SDHAF1 antibody - C-terminal
    See all SDHAF1 primary antibodies
  • Description

    Goat polyclonal to SDHAF1 - C-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey, Gorilla, Common marmoset
  • Immunogen

    Synthetic peptide corresponding to Human SDHAF1 aa 102-115 (C terminal) (Cysteine residue). (NP_001036096.1).
    Sequence:

    HDSTGAPETRPDGR


    Database link: A6NFY7

  • Positive control

    • Human Cerebellum lysate; human placenta tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab219764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 13 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II.
  • Tissue specificity

    Ubiquitously expressed.
  • Pathway

    Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
  • Involvement in disease

    Mitochondrial complex II deficiency
  • Sequence similarities

    Belongs to the complex I LYR family. SDHAF1 subfamily.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • LYR motif containing 8 antibody
    • LYR motif-containing protein 8 antibody
    • LYRM8 antibody
    • mitochondrial antibody
    • SDH assembly factor 1 antibody
    • SDHAF1 antibody
    • SDHF1_HUMAN antibody
    • Succinate dehydrogenase assembly factor 1 antibody
    • Succinate dehydrogenase assembly factor 1 mitochondrial antibody
    • Succinate dehydrogenase complex assembly factor 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling SDHAF1 with ab219764 at 5 µg/ml.

  • Anti-SDHAF1 antibody - C-terminal (ab219764) at 0.3 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 13 kDa



    Primary antibody incubation was 1 hour.

References

ab219764 has not yet been referenced specifically in any publications.

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