Key features and details
- Goat polyclonal to SDHAF1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SDHAF1 antibody - C-terminal
See all SDHAF1 primary antibodies
DescriptionGoat polyclonal to SDHAF1 - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Monkey, Gorilla, Common marmoset
- Human Cerebellum lysate; human placenta tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 13 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPlays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II.
Tissue specificityUbiquitously expressed.
PathwayCofactor biosynthesis; iron-sulfur cluster biosynthesis.
Involvement in diseaseMitochondrial complex II deficiency
Sequence similaritiesBelongs to the complex I LYR family. SDHAF1 subfamily.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- LYR motif containing 8 antibody
- LYR motif-containing protein 8 antibody
- LYRM8 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling SDHAF1 with ab219764 at 5 µg/ml.
Anti-SDHAF1 antibody - C-terminal (ab219764) at 0.3 µg/ml + Human Cerebellum lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 13 kDa
Primary antibody incubation was 1 hour.
ab219764 has not yet been referenced specifically in any publications.