Recombinant
RabMAb

Recombinant Anti-SDHAF1 antibody [EPR13380] (ab185222)

Overview

  • Product name

    Anti-SDHAF1 antibody [EPR13380]
    See all SDHAF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13380] to SDHAF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SDHAF1 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: A6NFY7

  • Positive control

    • Jurkat, MCF7 and 293 cell lysates; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.01% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13380
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab185222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 13 kDa).

This product detects the target protein in mitochondrial lysates (17 kDa). In our hands we are unable to detect the target protein in whole cell lysates.

ICC/IF 1/100.

Target

  • Function

    Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II.
  • Tissue specificity

    Ubiquitously expressed.
  • Pathway

    Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
  • Involvement in disease

    Mitochondrial complex II deficiency
  • Sequence similarities

    Belongs to the complex I LYR family. SDHAF1 subfamily.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • LYR motif containing 8 antibody
    • LYR motif-containing protein 8 antibody
    • LYRM8 antibody
    • mitochondrial antibody
    • SDH assembly factor 1 antibody
    • SDHAF1 antibody
    • SDHF1_HUMAN antibody
    • Succinate dehydrogenase assembly factor 1 antibody
    • Succinate dehydrogenase assembly factor 1 mitochondrial antibody
    • Succinate dehydrogenase complex assembly factor 1 antibody
    see all

Images

  • Immunofluorescent analysis of 293 cells (4% paraformaldehyde-fixed) labeling SDHAF1 with ab185222 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and Counterstained with DAPI (blue).

  • All lanes : Anti-SDHAF1 antibody [EPR13380] (ab185222) at 1/1000 dilution

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : MCF7 mitochondria fraction lysate
    Lane 3 : MCF7 mitochondria control lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 13 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

References

ab185222 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab185222.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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