Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13380] to SDHAF1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-SDHAF1 antibody [EPR13380]
See all SDHAF1 primary antibodies
DescriptionRabbit monoclonal [EPR13380] to SDHAF1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human SDHAF1 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: A6NFY7
- Jurkat, MCF7 and 293 cell lysates; 293 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 13 kDa).
This product detects the target protein in mitochondrial lysates (17 kDa). In our hands we are unable to detect the target protein in whole cell lysates.
FunctionPlays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II.
Tissue specificityUbiquitously expressed.
PathwayCofactor biosynthesis; iron-sulfur cluster biosynthesis.
Involvement in diseaseMitochondrial complex II deficiency
Sequence similaritiesBelongs to the complex I LYR family. SDHAF1 subfamily.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- LYR motif containing 8 antibody
- LYR motif-containing protein 8 antibody
- LYRM8 antibody
Immunofluorescent analysis of 293 cells (4% paraformaldehyde-fixed) labeling SDHAF1 with ab185222 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and Counterstained with DAPI (blue).
All lanes : Anti-SDHAF1 antibody [EPR13380] (ab185222) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate
Lane 2 : MCF7 mitochondria fraction lysate
Lane 3 : MCF7 mitochondria control lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking and dilution buffer: 5% NFDM/TBST.
ab185222 has not yet been referenced specifically in any publications.