• Product name
  • Description
    Goat polyclonal to SDHB
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 271/280 of Human SDHB

  • Positive control
    • Human Liver lysate



Our Abpromise guarantee covers the use of ab39988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:8,000
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa. (predicted molecular weight: 32 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
    • Pathway
      Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
    • Involvement in disease
      Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
      Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors.
      Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
      Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.
    • Sequence similarities
      Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
      Contains 1 2Fe-2S ferredoxin-type domain.
      Contains 1 4Fe-4S ferredoxin-type domain.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CWS2 antibody
      • DHSB_HUMAN antibody
      • FLJ92337 antibody
      • Ip antibody
      • Iron sulfur subunit antibody
      • Iron sulfur subunit of complex II antibody
      • Iron-sulfur subunit of complex II antibody
      • mitochondrial antibody
      • PGL 4 antibody
      • PGL4 antibody
      • SDH 1 antibody
      • SDH antibody
      • SDH1 antibody
      • SDH2 antibody
      • SDH2, homolog of antibody
      • SdhB antibody
      • SDHIP antibody
      • Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody
      • Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody
      • Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody
      • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody
      • Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody
      • Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody
      • Succinate dehydrogenase complex iron sulfur subunit B antibody
      • Succinate dehydrogenase complex subunit B iron sulfur antibody
      • Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody
      • succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody
      • Succinate dehydrogenase iron sulfur protein antibody
      see all


    • Anti-SDHB antibody (ab39988) at 0.003 µg/ml (for 1 hour) + Human Liver lysate in RIPA buffer at 35 µg

      Developed using the ECL technique.

      Predicted band size: 32 kDa
      Observed band size: 28 kDa
      why is the actual band size different from the predicted?


    ab39988 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As


    Thank you for your call yesterday and for your patience. I have heard back from the lab, and unfortunately there are not any more images showing results with different exposure times. I did receive the following information about the Western blot image on the datasheet: "Samples were run at 200V constant on a 12% acrylamide SDS-PAGE mini gel. Before loading samples had 5% (v/v) 2-ME added and were boiled for 3 minutes. Exposure was 1min (autoradiography film)." As we discussed, we would recommend using less protein and diluting the antibody more in order to reduce any non-specific bands that are showing on the blot. We also have the blocking peptide available for this antibody, ab45793, which can be used in a blocking assay to verify specificity of bands. Please keep me updated on the results of any further testing with the antibody. If the bands do not diminish, let me know and I will be happy to help you further. If you have any questions please don't hesitate to contact me.

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