Overview

  • Product name
    Anti-SDHB antibody [EPR10880]
    See all SDHB primary antibodies
  • Description
    Rabbit monoclonal [EPR10880] to SDHB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SDHB aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P21912

  • Positive control
    • WB: HepG2, A431 and Jurkat cell lysates and human fetal heart, mouse brain and rat brain tissue lysates. IHC-P: Human caratoid paraganglioma, human mesenchymoma, human kidney, human liver, mouse kidney and rat kidney tissues. Flow Cyt: Jurkat and A431 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 32 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/2000.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
    • Pathway
      Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
    • Involvement in disease
      Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
      Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors.
      Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
      Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.
    • Sequence similarities
      Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
      Contains 1 2Fe-2S ferredoxin-type domain.
      Contains 1 4Fe-4S ferredoxin-type domain.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CWS2 antibody
      • DHSB_HUMAN antibody
      • FLJ92337 antibody
      • Ip antibody
      • Iron sulfur subunit antibody
      • Iron sulfur subunit of complex II antibody
      • Iron-sulfur subunit of complex II antibody
      • mitochondrial antibody
      • PGL 4 antibody
      • PGL4 antibody
      • SDH 1 antibody
      • SDH antibody
      • SDH1 antibody
      • SDH2 antibody
      • SDH2, homolog of antibody
      • SdhB antibody
      • SDHIP antibody
      • Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody
      • Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody
      • Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody
      • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody
      • Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody
      • Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody
      • Succinate dehydrogenase complex iron sulfur subunit B antibody
      • Succinate dehydrogenase complex subunit B iron sulfur antibody
      • Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody
      • succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody
      • Succinate dehydrogenase iron sulfur protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SDHB with purified ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labeling SDHB with unpurified ab175225 at a dilution of 1/100.

    • All lanes : Anti-SDHB antibody [EPR10880] (ab175225) at 1/100000 dilution (purified)

      Lane 1 : HepG2 whole cell lysate
      Lane 2 : Jurkat whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking and dilution buffer: 5% NFDM/TBST
    • All lanes : Anti-SDHB antibody [EPR10880] (ab175225) at 1/100000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : Mouse spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking and dilution buffer: 5% NFDM/TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human mesenchymoma tissue labelling SDHB with purified ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling SDHB with purified ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling SDHB with purified ab175225 at a dilution of 1/2000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Flow Cytometry analysis of A431 cells labelling SDHB with purified ab175225 at a dilution of 1/200 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • ab175225 (purified) at a dilution of 1/60 immunoprecipitating SDHB in Jurkat whole cell lysate.

      Lane 1 (input): Jurkat whole cell lysate (10µg)

      Lane 2 (+): ab175225 + Jurkat whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab175225 in Jurkat whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-SDHB antibody [EPR10880] (ab175225) at 1/50000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Fetal heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 32 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labeling SDHB with unpurified ab175225 at a dilution of 1/100.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human carotid paraganglioma tissue labeling SDHB with unpurified ab175225 at a dilution of 1/100.

    • Flow cytometric analysis of permeabilized Jurkat cells labeling SDHB with unpurified ab175225 at a dilution of 1/10 (red) compared to a rabbit IgG negative control (green).

    References

    This product has been referenced in:
    • Mot AI  et al. Circumventing the Crabtree Effect: A method to induce lactate consumption and increase oxidative phosphorylation in cell culture. Int J Biochem Cell Biol 79:128-138 (2016). Read more (PubMed: 27590850) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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