Overview

  • Product name

  • Description

    Rabbit polyclonal to SDHC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SDHC aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: Q99643

  • Positive control

    • This antibody gave a positive signal in a nuclear extract from Jurkat cells, and in the following whole cell lysates: THP1; U937; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab129736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Target

  • Function

    Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
  • Pathway

    Carbohydrate metabolism; tricarboxylic acid cycle.
  • Involvement in disease

    Defects in SDHC are the cause of hereditary paragangliomas type 3 (PGL3) [MIM:605373]; also known as autosomal dominant non-chromaffin paragangliomas type 3. Non-chromaffin paragangliomas are usually benign, neural crest derived tumors of parasympathetic ganglia.
    Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also known as Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
  • Sequence similarities

    Belongs to the cytochrome b560 family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CYB560 antibody
    • C560_HUMAN antibody
    • CYBL antibody
    • Cytochrome B large subunit of complex II antibody
    • Integral membrane protein CII-3 antibody
    • mitochondrial antibody
    • PGL3 antibody
    • QPs-1 antibody
    • QPs1 antibody
    • SDH3 antibody
    • sdhC antibody
    • succinate dehydrgenase cytochrome b antibody
    • succinate dehydrogenase 3, integral membrane subunit antibody
    • Succinate dehydrogenase complex subunit C antibody
    • Succinate dehydrogenase complex subunit C integral membrane protein 15kDa antibody
    • Succinate dehydrogenase cytochrome b560 subunit antibody
    • Succinate dehydrogenase cytochrome b560 subunit mitochondrial antibody
    • Succinate dehydrogenase cytochrome b560 subunit, mitochondrial precursor antibody
    • Succinate-ubiquinone oxidoreductase cytochrome B large subunit antibody
    see all

Images

  • All lanes : Anti-SDHC antibody (ab129736) at 1 µg/ml

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa
    Additional bands at: 13 kDa (possible non-specific binding), 140 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129736 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab129736 has not yet been referenced specifically in any publications.

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