• Product name

  • Description

    Rabbit polyclonal to SDHD
  • Host species

  • Specificity

    ab189945 will detect three isoforms of SDHD.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SDHD (internal sequence). The exact sequence is proprietary. NCBI Accession No. NP_002993.1. The synthetic peptide corresponds to 15 amino acids.
    Database link: O14521

  • Positive control

    • Human kidney tissue; mouse EL4 cell lysate and cells.



Our Abpromise guarantee covers the use of ab189945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P Use a concentration of 2.5 µg/ml.


  • Function

    Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
  • Pathway

    Carbohydrate metabolism; tricarboxylic acid cycle.
  • Involvement in disease

    Defects in SDHD are a cause of hereditary paragangliomas type 1 (PGL1) [MIM:168000]; also known as familial non-chromaffin paragangliomas type 1. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL1 is a rare autosomal dominant disorder which is characterized by the development of mostly benign, highly vascular, slowly growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors. Penetrance of PGL1 is incomplete when the disease is transmitted through fathers. No disease phenotype is transmitted maternally.
    Defects in SDHD are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
    Defects in SDHD may be a cause of susceptibility to intestinal carcinoid tumor (ICT) [MIM:114900]. A yellow, well-differentiated, circumscribed tumor that arises from enterochromaffin cells in the small intestine or, less frequently, in other parts of the gastrointestinal tract.
    Defects in SDHD are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
    Defects in SDHD are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.
  • Sequence similarities

    Belongs to the CybS family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CBT1 antibody
    • CII 4 antibody
    • CII-4 antibody
    • CII4 antibody
    • CWS3 antibody
    • CybS antibody
    • DHSD_HUMAN antibody
    • mitochondrial antibody
    • OTTHUMP00000234720 antibody
    • OTTHUMP00000234721 antibody
    • OTTHUMP00000234722 antibody
    • OTTHUMP00000234723 antibody
    • OTTHUMP00000234724 antibody
    • OTTHUMP00000234725 antibody
    • OTTHUMP00000234726 antibody
    • PGL antibody
    • PGL1 antibody
    • QPs3 antibody
    • SDH4 antibody
    • sdhD antibody
    • Succinate dehydrogenase [ubiquinone] cytochrome b small subunit antibody
    • Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial antibody
    • Succinate dehydrogenase complex subunit D antibody
    • Succinate dehydrogenase complex, subunit D, integral membrane protein antibody
    • Succinate dehydrogenase ubiquinone cytochrome B small subunit antibody
    • Succinate ubiquinone oxidoreductase cytochrome b small subunit antibody
    • Succinate ubiquinone reductase membrane anchor subunit antibody
    • Succinate-ubiquinone oxidoreductase cytochrome b small subunit antibody
    • Succinate-ubiquinone reductase membrane anchor subunit antibody
    see all


  • Lane 1 : Anti-SDHD antibody (ab189945) at 1 µg/ml
    Lane 2 : Anti-SDHD antibody (ab189945) at 2 µg/ml

    All lanes : mouse EL4 cell lysate

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling SDHD using ab189945 at 2.5 µg/ml.


This product has been referenced in:

  • Zhang T  et al. SDHD Promoter Mutations Ablate GABP Transcription Factor Binding in Melanoma. Cancer Res 77:1649-1661 (2017). Read more (PubMed: 28108517) »
  • Jiang Q  et al. A novel germline mutation in SDHA identified in a rare case of gastrointestinal stromal tumor complicated with renal cell carcinoma. Int J Clin Exp Pathol 8:12188-97 (2015). Read more (PubMed: 26722403) »
See all 2 Publications for this product

Customer reviews and Q&As

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Western blot
Mouse Tissue lysate - other (Mitochondria isolated brain tissue)
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol, 10 min at +55C denaturation. Immobilon-FL transfer membranes (Millipore), overnight transfer at +4C.)
Loading amount
15 µg
Mitochondria isolated brain tissue
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

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Submitted Oct 17 2018

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