Anti-SDS3 antibody (ab3740)
Key features and details
- Goat polyclonal to SDS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SDS3 antibody
See all SDS3 primary antibodies -
Description
Goat polyclonal to SDS3 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide (Human) - which represented a portion of human Suppressor of Defective Silencing 3 encoded in part by exons 7 and 8.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Rabbit
Chicken
Guinea pig
Cow
Dog
Turkey
Pig
Xenopus laevis
Chimpanzee
Zebrafish
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 45 kDa. |
Target
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Function
Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways (By similarity). May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. -
Tissue specificity
Expressed in various cancer cell ines. -
Sequence similarities
Belongs to the SDS3 family. -
Domain
The C-terminus is involved in transcriptional repression by HDAC-independent mechanisms. - Information by UniProt
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Database links
- Entrez Gene: 64426 Human
- Entrez Gene: 445099 Zebrafish
- Omim: 608250 Human
- SwissProt: Q9H7L9 Human
- SwissProt: Q803C3 Zebrafish
- Unigene: 416630 Human
- Unigene: 602312 Human
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Alternative names
- 2400003N08Rik antibody
- 2410008L21Rik antibody
- 45 kDa Sin3 associated polypeptide antibody
see all
Images
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Western blot - Anti-SDS3 antibody (ab3740)
Sample: HeLa whole cell lysate (50
µ g/lane)Antibody: Affinity purified Goat anti-SDS 3 (ab3740) used at the concentrations indicated.
Detection: ECL exposed for 2 minutes.
Sample: HeLa whole cell lysate (50
µg/lane) Antibody: Affinity purified Goat anti-SDS 3 (ab3740) used at the concentrations indicated.
Detection: ECL exposed for 2 minutes.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab3740 has been referenced in 2 publications.
- Ramakrishna S et al. Hyaluronan Binding Motifs of USP17 and SDS3 Exhibit Anti-Tumor Activity. PLoS One 7:e37772 (2012). WB ; Human . PubMed: 22662218
- Ramakrishna S et al. K63 specific deubiquitination of SDS3 by USP17 regulates HDAC activity. J Biol Chem : (2011). IP ; Human . PubMed: 21239494