Anti-SDS3 antibody (ab3740)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-SDS3 antibody
See all SDS3 primary antibodies -
DescriptionGoat polyclonal to SDS3
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Host speciesGoat
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide (Human) - which represented a portion of human Suppressor of Defective Silencing 3 encoded in part by exons 7 and 8.
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Positive control
- HeLa cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 45 kDa. | |
| IP | Use at an assay dependent dilution. PubMed: 21239494 |
Target
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FunctionRegulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways (By similarity). May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
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Tissue specificityExpressed in various cancer cell ines.
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Sequence similaritiesBelongs to the SDS3 family.
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DomainThe C-terminus is involved in transcriptional repression by HDAC-independent mechanisms.
- Information by UniProt
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Database links
- Entrez Gene: 64426 Human
- Entrez Gene: 71954 Mouse
- Entrez Gene: 445099 Zebrafish
- Omim: 608250 Human
- SwissProt: Q9H7L9 Human
- SwissProt: Q8BR65 Mouse
- SwissProt: Q803C3 Zebrafish
- Unigene: 416630 Human
see all -
Alternative names
- 2400003N08Rik antibody
- 2410008L21Rik antibody
- 45 kDa Sin3 associated polypeptide antibody
see all
Images
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Western blot - Anti-SDS3 antibody (ab3740)Sample: HeLa whole cell lysate (50
µ g/lane)Antibody: Affinity purified Goat anti-SDS 3 (ab3740) used at the concentrations indicated.
Detection: ECL exposed for 2 minutes.
Sample: HeLa whole cell lysate (50
µg/lane) Antibody: Affinity purified Goat anti-SDS 3 (ab3740) used at the concentrations indicated.
Detection: ECL exposed for 2 minutes.
References
This product has been referenced in:
- Ramakrishna S et al. Hyaluronan Binding Motifs of USP17 and SDS3 Exhibit Anti-Tumor Activity. PLoS One 7:e37772 (2012). WB ; Human . Read more (PubMed: 22662218) »
- Ramakrishna S et al. K63 specific deubiquitination of SDS3 by USP17 regulates HDAC activity. J Biol Chem : (2011). IP ; Human . Read more (PubMed: 21239494) »
