Product nameAnti-SDS3 antibody [EPR15000]
See all SDS3 primary antibodies
DescriptionRabbit monoclonal [EPR15000] to SDS3
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human SDS3 aa 1-150. The exact sequence is proprietary.
Database link: Q9H7L9
- Raji, HeLa, K562 and Molt-4 cell lysates; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRegulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways (By similarity). May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
Tissue specificityExpressed in various cancer cell ines.
Sequence similaritiesBelongs to the SDS3 family.
DomainThe C-terminus is involved in transcriptional repression by HDAC-independent mechanisms.
- Information by UniProt
- 2400003N08Rik antibody
- 2410008L21Rik antibody
- 45 kDa Sin3 associated polypeptide antibody
All lanes : Anti-SDS3 antibody [EPR15000] (ab184555) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Molt-4 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SDS3 with ab184555 at 1/250 dilution (red) compared to a Rabbit monoclonal isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.
This product has been referenced in:
- Banks CAS et al. Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly. Sci Rep 8:13712 (2018). Read more (PubMed: 30209338) »
- Banks CAS et al. A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry. Mol Cell Proteomics 17:1432-1447 (2018). Read more (PubMed: 29599190) »