Recombinant Anti-SDS3 antibody [EPR15000] (ab184555)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15000] to SDS3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SDS3 antibody [EPR15000]
See all SDS3 primary antibodies -
Description
Rabbit monoclonal [EPR15000] to SDS3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Raji, HeLa, K562 and Molt-4 cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15000 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/250.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).
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Notes |
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Flow Cyt (Intra)
1/250. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways (By similarity). May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. -
Tissue specificity
Expressed in various cancer cell ines. -
Sequence similarities
Belongs to the SDS3 family. -
Domain
The C-terminus is involved in transcriptional repression by HDAC-independent mechanisms. - Information by UniProt
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Database links
- Entrez Gene: 64426 Human
- Entrez Gene: 71954 Mouse
- Entrez Gene: 360819 Rat
- Omim: 608250 Human
- SwissProt: Q9H7L9 Human
- SwissProt: Q8BR65 Mouse
- Unigene: 416630 Human
- Unigene: 602312 Human
see all -
Alternative names
- 2400003N08Rik antibody
- 2410008L21Rik antibody
- 45 kDa Sin3 associated polypeptide antibody
see all
Images
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All lanes : Anti-SDS3 antibody [EPR15000] (ab184555) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Molt-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SDS3 with ab184555 at 1/250 dilution (red) compared to a Rabbit monoclonal isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibodyat 1/150 dilution.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab184555 has been referenced in 2 publications.
- Banks CAS et al. Differential HDAC1/2 network analysis reveals a role for prefoldin/CCT in HDAC1/2 complex assembly. Sci Rep 8:13712 (2018). PubMed: 30209338
- Banks CAS et al. A Structured Workflow for Mapping Human Sin3 Histone Deacetylase Complex Interactions Using Halo-MudPIT Affinity-Purification Mass Spectrometry. Mol Cell Proteomics 17:1432-1447 (2018). PubMed: 29599190