Sea Block Buffer (ab166951)
Overview
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Product name
Sea Block Buffer -
Tested applications
Suitable for: Blocking, ELISAmore details -
General notes
Sea Block Buffer is a non-mammalian blocking reagent supplied in PBS for immunoassays involving membrane or other solid phase material. This new reagent imparts superior stability and flow characteristics, while utilizing proteins that do not interact with mammalian antibodies.
Numerous solutions containing proteins are used and recommended for blocking of excess binding sites in ELISA, Western Blotting, Immunohistochemistry and other immunochemical applications. Sea Block Buffer utilizes proteins that do not interact substantially with mammalian antibodies and therefore will display little or no background.
How to Use:
1. Add the blocking solution to the ELISA wells, blotting membrane or immuhistochemical slide.
2. Incubate 30 minutes to 2 hours at either room temperature or 37°C.
3. Continue with other reagents after the blocking according to your own protocol.NOTE: Using this blocker as a diluent with HRP conjugates may decrease your signal since this blocker contains sodium azide.
Purity: Whole antiserum
Properties
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Form
Liquid -
Storage instructions
Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Research areas
Applications
Our Abpromise guarantee covers the use of ab166951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Blocking | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. This blocking solution should be used directly in your ELISA plates. If a decrease in signal is noted, further dilutions of the solution may restore the signal. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab166951 has been referenced in 1 publication.
- Chen CY et al. Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure. Nat Med 24:1225-1233 (2018). PubMed: 29892068
