Overview

  • Product name
    Sea Block Buffer
  • Tested applications
    Suitable for: Blocking, ELISAmore details
  • General notes

    Sea Block Buffer is a non-mammalian blocking reagent supplied in PBS for immunoassays involving membrane or other solid phase material. This new reagent imparts superior stability and flow characteristics, while utilizing proteins that do not interact with mammalian antibodies.

    Numerous solutions containing proteins are used and recommended for blocking of excess binding sites in ELISA, Western Blotting, Immunohistochemistry and other immunochemical applications. Sea Block Buffer utilizes proteins that do not interact substantially with mammalian antibodies and therefore will display little or no background.

    How to Use:
    1. Add the blocking solution to the ELISA wells, blotting membrane or immuhistochemical slide.
    2. Incubate 30 minutes to 2 hours at either room temperature or 37°C.
    3. Continue with other reagents after the blocking according to your own protocol.

    NOTE: Using this blocker as a diluent with HRP conjugates may decrease your signal since this blocker contains sodium azide.

    Purity: Whole antiserum

Properties

Applications

Our Abpromise guarantee covers the use of ab166951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. This blocking solution should be used directly in your ELISA plates. If a decrease in signal is noted, further dilutions of the solution may restore the signal.

References

ab166951 has not yet been referenced specifically in any publications.

Publishing research using ab166951? Please let us know so that we can cite the reference in this datasheet.

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I have a small question regarding the choice of Sea Block Buffer (ab166951) or Aqua Block Buffer (ab166952) for use in ELISA. What is the difference between them and what should be the decisive factor of choice?




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The Sea Block Buffer (ab166951) has been optimised for ELISA whereas the Aqua block buffer (ab166952) is specifically formulated for Western blot.


Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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