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Sea Block serum free -Tris is a proprietary blocking reagent supplied in TBS for immunoassays involving membrane or other solid phase material. This new reagent imparts superior stability and flow characteristics, while utilizing proteins that do not interact with mammalian antibodies.
Numerous solutions containing proteins are used and recommended for blocking of excess binding sites in ELISA, Western Blotting, Immunohistochemistry and other immunochemical applications. Sea Block serum free -PBS utilizes proteins that do not interact substantially with mammalian antibodies and therefore will display little or no background.
How to Use:
1. Add the blocking solution to the ELISA wells, blotting membrane or immuhistochemical slide.
2. Incubate 30 minutes to 2 hours at either room temperature or 37°C.
3. Continue with other reagents after the blocking according to your own protocol.
NOTE: Using this blocker as a diluent with HRP conjugates will not decrease your signal since this blocker contains no sodium azide. This product is a non-mammalian blocking reagent.
Our Abpromise guarantee covers the use of ab166956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. This blocking solution should be used directly in your ELISA plates. If a decrease in signal is noted, further dilutions of the solution may restore the signal.|
|WB||Use at an assay dependent concentration. This blocking solution should be used directly in your blots. If a decrease in signal is noted, further dilutions of the solution may restore the signal.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.|
ab166956 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"