Product nameSea Grow
Sea Grow is derived from aseptically drawn whole blood and plasma products from salmonids. It works well for fish and insect cell culture when used as a media additive.
Endotoxin <0.5 EU/ml
Sea Grow has not been heat-treated, and will gel if heated alone. To prevent gelling, add a minimum of an equal volume of defined media to Sea Grow before heating to 55ºC for 30 minutes. Depending on your specific application, addition of at least twice the volume of defined media may be necessary to prevent gelling. Alternatively, users may dilute Sea Grow to the final working concentration prior to heating.
For Fish and Insect Cells
1. Try Sea Grow at the 2.5% or 5% level if you have been using 10% or more FBS.
2. Heat-treatment may not be necessary for some cell lines.
3. Sterile filtration to 0.2 micron after combining with defined media is recommended prior to use in culture
Sea Grow does not contain any mammalian proteins. Although the immune system of fish is similar to that of other vertebrates, fish antibodies are unlike those of mammals and do not cross-react.
Tested applicationsSuitable for: IHC-Fr, Blocking, IHC-P, WBmore details
Storage instructionsStore at -80°C. Please refer to protocols.
Components 10 ml Sea Grow 3 x 10ml
Our Abpromise guarantee covers the use of ab170731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
ab170731 has not yet been referenced specifically in any publications.