Key features and details
- Assay type: Quantitative
- Detection method: Luminescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media
Product nameSEAP Reporter Gene Assay Kit (Luminescence)
Sample typeCell culture media
Assay time1h 00m
Species reactivityReacts with: Mammals, Other species
SEAP Reporter Gene Assay Kit (Luminescence) (ab133077) provides a simple chemiluminescence method for the sensitive quantitation of SEAP (secreted alkaline phosphatase) in conditioned cell culture medium from transfected cells. The assay can detect SEAP activity in the microunits/well (mU/ml) range. The kit includes enough reagents to run three 96-well plates. The assay is easy to perform and can be completed within one hour.
Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. SEAP activity in the culture medium is directly proportional to changes in intracellular concentrations of SEAP mRNA and protein. In addition, the kinetics of gene expression can be studied using the same cultures by repeatedly collecting culture medium at different time points. The intact cells can be used for further analysis of RNA or protein expression.
SEAP activity was first measured using the chromogenic alkaline phosphatase substrate p-nitrophenyl phosphate (pNPP). Today, the most sensitive SEAP assays employ chemiluminescent alkaline phosphatase substrates such as the 1,2-dioxetane CSPD. Chemiluminescent detection of SEAP is fast, easy to perform, and sensitive.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 300 tests 96-Well Solid Plate (white) with lid 3 units Cell-Based Alkaline Phosphatase Standard 1 x 200µl SEAP Substrate (Luminescence) 1 x 15ml
ab133077 has not yet been referenced specifically in any publications.