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SEAP Reporter Gene Assay Kit (Luminescence) (ab133077)

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Functional Studies - SEAP Reporter Gene Assay Kit (Luminescence) (ab133077)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Luminescent
    • Platform: Microplate reader
    • Assay time: 1 hr
    • Sample type: Cell culture media

    Overview

    • Product name

      SEAP Reporter Gene Assay Kit (Luminescence)
    • Detection method

      Luminescent
    • Sample type

      Cell culture media
    • Assay type

      Quantitative
    • Assay time

      1h 00m
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      SEAP Reporter Gene Assay Kit (Luminescence) (ab133077) provides a simple chemiluminescence method for the sensitive quantitation of SEAP (secreted alkaline phosphatase) in conditioned cell culture medium from transfected cells. The assay can detect SEAP activity in the microunits/well (mU/ml) range. The kit includes enough reagents to run three 96-well plates. The assay is easy to perform and can be completed within one hour.

    • Notes

      Secreted alkaline phosphatase (SEAP) is commonly used as a reporter of gene expression. Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. SEAP activity in the culture medium is directly proportional to changes in intracellular concentrations of SEAP mRNA and protein. In addition, the kinetics of gene expression can be studied using the same cultures by repeatedly collecting culture medium at different time points. The intact cells can be used for further analysis of RNA or protein expression.

      SEAP activity was first measured using the chromogenic alkaline phosphatase substrate p-nitrophenyl phosphate (pNPP). Today, the most sensitive SEAP assays employ chemiluminescent alkaline phosphatase substrates such as the 1,2-dioxetane CSPD. Chemiluminescent detection of SEAP is fast, easy to perform, and sensitive.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 300 tests
      96-Well Solid Plate (white) with lid 3 units
      Cell-Based Alkaline Phosphatase Standard 1 x 200µl
      SEAP Substrate (Luminescence) 1 x 15ml
    • Research areas

      • Kits/ Lysates/ Other
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    Images

    • Functional Studies - SEAP Reporter Gene Assay Kit (Luminescence) (ab133077)
      Functional Studies - SEAP Reporter Gene Assay Kit (Luminescence) (ab133077)
      Cell-Based Alkaline Phosphatase Standard curve

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab133077? Please let us know so that we can cite the reference in this datasheet.

    ab133077 has not yet been referenced specifically in any publications.

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