Overview

  • Product name

    Anti-SEBOX antibody
  • Description

    Rabbit polyclonal to SEBOX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 148-216 of Human SEBOX (UniProt: Q9HB31).

  • Positive control

    • Human bone marrow tissue.
  • General notes

    The antibody solution should be gently mixed before use

Properties

Applications

Our Abpromise guarantee covers the use of ab150935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Probable transcription factor involved in the control of specification of mesoderm and endoderm.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Homeobox OG 9 antibody
    • OG9 antibody
    • OG9X antibody
    • SEBOX homeobox antibody
    • Skin-, embryo-, brain- and oocyte-specific homeobox antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling SEBOX with ab150935 at 1/50 dilution.
  • IHC-P image of SEBOX staining with ab150935 on tissue sections from adult marmoset ovary. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab150935 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

References

ab150935 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (Adult Ovary)
Specification
Adult Ovary
Permeabilization
No
Fixative
Formaldehyde

Dr. 煜欽 林

Verified customer

Submitted Aug 01 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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