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    sec13l1-antibody-ab168824.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Nuclear Pore
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Anti-SEC13L1 antibody (ab168824)

  • Datasheet
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Western blot - Anti-SEC13L1 antibody (ab168824)
  • Immunoprecipitation - Anti-SEC13L1 antibody (ab168824)

Key features and details

  • Rabbit polyclonal to SEC13L1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human SEC13L1 protein (ab105635)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SEC13L1 antibody
    See all SEC13L1 primary antibodies
  • Description

    Rabbit polyclonal to SEC13L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Dog, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 270-320 of Human SEC13L1 (NP_899195.1).

  • Positive control

    • 293T, HeLa, Jurkat, mouse TCMK1 and mouse NIH 3T3 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab168824 was affinity purified using an epitope specific to SEC13L1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human SEC13L1 protein (ab105635)
  • Related Products

    • Recombinant Human SEC13L1 protein (ab105635)

Applications

Our Abpromise guarantee covers the use of ab168824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles.
  • Sequence similarities

    Belongs to the WD repeat SEC13 family.
    Contains 6 WD repeats.
  • Cellular localization

    Cytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. In interphase, localizes at both sides of the NPC.
  • Target information above from: UniProt accession P55735 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 460167 Chimpanzee
    • Entrez Gene: 607653 Dog
    • Entrez Gene: 101153194 Gorilla
    • Entrez Gene: 6396 Human
    • Entrez Gene: 110379 Mouse
    • Entrez Gene: 100451553 Orangutan
    • Entrez Gene: 697339 Rhesus monkey
    • Omim: 600152 Human
    • SwissProt: P55735 Human
    • SwissProt: Q9D1M0 Mouse
    • Unigene: 166924 Human
    • Unigene: 29296 Mouse
    see all
  • Form

    It is a constituent of the endoplasmic reticulum and the nuclear pore complex. Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Cellular location: Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus.
  • Alternative names

    • D3S1231E antibody
    • Npp 20 antibody
    • Npp20 antibody
    • Protein SEC13 homolog antibody
    • SEC13 (S. cerevisiae) like 1 antibody
    • Sec13 antibody
    • SEC13 homolog (S. cerevisiae) antibody
    • SEC13 homolog antibody
    • SEC13 like 1 (S. cerevisiae) antibody
    • SEC13 like 1 isoform antibody
    • SEC13 like protein 1 antibody
    • SEC13 protein antibody
    • SEC13 related protein antibody
    • SEC13-like protein 1 antibody
    • SEC13-related protein antibody
    • SEC13_HUMAN antibody
    • SEC13R antibody
    see all

Images

  • Western blot - Anti-SEC13L1 antibody (ab168824)
    Western blot - Anti-SEC13L1 antibody (ab168824)
    All lanes : Anti-SEC13L1 antibody (ab168824) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 35 kDa


    Exposure time: 10 seconds
  • Immunoprecipitation - Anti-SEC13L1 antibody (ab168824)
    Immunoprecipitation - Anti-SEC13L1 antibody (ab168824)
    Detection of SEC13L1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168824 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab168824? Please let us know so that we can cite the reference in this datasheet.

    ab168824 has been referenced in 1 publication.

    • Fan J  et al. cTAGE5 deletion in pancreatic ß cells impairs proinsulin trafficking and insulin biogenesis in mice. J Cell Biol 216:4153-4164 (2017). PubMed: 29133483

    Customer reviews and Q&As

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