Anti-SEC13L1 antibody (ab168824)
Key features and details
- Rabbit polyclonal to SEC13L1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SEC13L1 antibody
See all SEC13L1 primary antibodies -
Description
Rabbit polyclonal to SEC13L1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Dog, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Bat
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 270-320 of Human SEC13L1 (NP_899195.1).
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Positive control
- 293T, HeLa, Jurkat, mouse TCMK1 and mouse NIH 3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab168824 was affinity purified using an epitope specific to SEC13L1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 35 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. -
Sequence similarities
Belongs to the WD repeat SEC13 family.
Contains 6 WD repeats. -
Cellular localization
Cytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. In interphase, localizes at both sides of the NPC. - Information by UniProt
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Database links
- Entrez Gene: 460167 Chimpanzee
- Entrez Gene: 607653 Dog
- Entrez Gene: 101153194 Gorilla
- Entrez Gene: 6396 Human
- Entrez Gene: 110379 Mouse
- Entrez Gene: 100451553 Orangutan
- Entrez Gene: 697339 Rhesus monkey
- Omim: 600152 Human
see all -
Form
It is a constituent of the endoplasmic reticulum and the nuclear pore complex. Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Cellular location: Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus. -
Alternative names
- D3S1231E antibody
- Npp 20 antibody
- Npp20 antibody
see all
Images
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Western blot - Anti-SEC13L1 antibody (ab168824)All lanes : Anti-SEC13L1 antibody (ab168824) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-SEC13L1 antibody (ab168824)Detection of SEC13L1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168824 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
References (1)
ab168824 has been referenced in 1 publication.
- Fan J et al. cTAGE5 deletion in pancreatic ß cells impairs proinsulin trafficking and insulin biogenesis in mice. J Cell Biol 216:4153-4164 (2017). PubMed: 29133483
