Anti-SEC13L1 antibody (ab168824)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SEC13L1 antibody
See all SEC13L1 primary antibodies -
Description
Rabbit polyclonal to SEC13L1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Dog, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Bat
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 270-320 of Human SEC13L1 (NP_899195.1).
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Positive control
- 293T, HeLa, Jurkat, mouse TCMK1 and mouse NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab168824 was affinity purified using an epitope specific to SEC13L1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab168824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 35 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. -
Sequence similarities
Belongs to the WD repeat SEC13 family.
Contains 6 WD repeats. -
Cellular localization
Cytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. In interphase, localizes at both sides of the NPC. - Information by UniProt
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Database links
- Entrez Gene: 460167 Chimpanzee
- Entrez Gene: 607653 Dog
- Entrez Gene: 101153194 Gorilla
- Entrez Gene: 6396 Human
- Entrez Gene: 110379 Mouse
- Entrez Gene: 100451553 Orangutan
- Entrez Gene: 697339 Rhesus monkey
- Omim: 600152 Human
see all -
Form
It is a constituent of the endoplasmic reticulum and the nuclear pore complex. Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Cellular location: Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus. -
Alternative names
- D3S1231E antibody
- Npp 20 antibody
- Npp20 antibody
see all
Images
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Western blot - Anti-SEC13L1 antibody (ab168824)All lanes : Anti-SEC13L1 antibody (ab168824) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-SEC13L1 antibody (ab168824)Detection of SEC13L1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168824 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
References
This product has been referenced in:
- Fan J et al. cTAGE5 deletion in pancreatic ß cells impairs proinsulin trafficking and insulin biogenesis in mice. J Cell Biol 216:4153-4164 (2017). Read more (PubMed: 29133483) »
