Anti-SEC14 like protein 2 antibody (ab236888)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SEC14 like protein 2 antibody -
Description
Rabbit polyclonal to SEC14 like protein 2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SEC14 like protein 2 aa 233-358.
Database link: O76054 -
Positive control
- WB: PC-3 whole cell lysate. IHC-P: Human prostate and colon cancer tissues. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab236888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/200. | |
| IHC-P | 1/200 - 1/500. | |
| WB | 1/500 - 1/5000. Predicted molecular weight: 46 kDa. |
Target
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Function
Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell. -
Tissue specificity
Widely expressed. Strong expression in liver, brain and prostate. -
Sequence similarities
Contains 1 CRAL-TRIO domain.
Contains 1 GOLD domain. -
Developmental stage
Low expression in fetal tissues. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in absence of alpha-tocopherol, and nuclear in presence of alpha-tocopherol. - Information by UniProt
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Database links
- Entrez Gene: 23541 Human
- Omim: 607558 Human
- SwissProt: O76054 Human
- Unigene: 335614 Human
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Alternative names
- Alpha tocopherol associated protein antibody
- Alpha-tocopherol-associated protein antibody
- C22orf6 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SEC14 like protein 2 antibody (ab236888)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SEC14 like protein 2 (green) using ab236888 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI (blue).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
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Western blot - Anti-SEC14 like protein 2 antibody (ab236888)Anti-SEC14 like protein 2 antibody (ab236888) at 1/500 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14 like protein 2 antibody (ab236888)Paraffin-embedded human prostate tissue stained for SEC14 like protein 2 using ab236888 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC14 like protein 2 antibody (ab236888)Paraffin-embedded human colon cancer tissue stained for SEC14 like protein 2 using ab236888 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
References
ab236888 has not yet been referenced specifically in any publications.
