Overview

  • Product name

    Anti-SEC14 like protein 2 antibody
  • Description

    Rabbit polyclonal to SEC14 like protein 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SEC14 like protein 2 aa 233-358.
    Database link: O76054

  • Positive control

    • WB: PC-3 whole cell lysate. IHC-P: Human prostate and colon cancer tissues. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab236888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/200 - 1/500.
WB 1/500 - 1/5000. Predicted molecular weight: 46 kDa.

Target

  • Function

    Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell.
  • Tissue specificity

    Widely expressed. Strong expression in liver, brain and prostate.
  • Sequence similarities

    Contains 1 CRAL-TRIO domain.
    Contains 1 GOLD domain.
  • Developmental stage

    Low expression in fetal tissues.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in absence of alpha-tocopherol, and nuclear in presence of alpha-tocopherol.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha tocopherol associated protein antibody
    • Alpha-tocopherol-associated protein antibody
    • C22orf6 antibody
    • hTAP antibody
    • KIAA1186 antibody
    • KIAA1658 antibody
    • MGC65053 antibody
    • OTTHUMP00000198972 antibody
    • OTTHUMP00000198973 antibody
    • OTTHUMP00000198974 antibody
    • OTTHUMP00000199021 antibody
    • S14L2_HUMAN antibody
    • SEC14 (S. cerevisiae) like 2 antibody
    • SEC14 like 2 (S. cerevisiae) antibody
    • SEC14 like protein 2 antibody
    • SEC14-like protein 2 antibody
    • Sec14l2 antibody
    • SPF antibody
    • Squalene transfer protein antibody
    • Supernatant protein factor antibody
    • TAP antibody
    • TAP1 antibody
    • tocopherol-associated protein antibody
    see all

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SEC14 like protein 2 (green) using ab236888 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI (blue).

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. 

  • Anti-SEC14 like protein 2 antibody (ab236888) at 1/500 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 46 kDa

  • Paraffin-embedded human prostate tissue stained for SEC14 like protein 2 using ab236888 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

     

  • Paraffin-embedded human colon cancer tissue stained for SEC14 like protein 2 using ab236888 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab236888 has not yet been referenced specifically in any publications.

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