Anti-SEC16A/SEC16 antibody (ab70722)
Key features and details
- Rabbit polyclonal to SEC16A/SEC16
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SEC16A/SEC16 antibody -
Description
Rabbit polyclonal to SEC16A/SEC16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Ferret, Rhesus monkey, Gorilla, Orangutan, Bat -
Immunogen
Synthetic peptide corresponding to Human SEC16A/SEC16.
Database link: O15027 -
Positive control
- Whole cell lysate from HeLa and 293T cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/2000 - 1/10000. Detects a band of approximately >250 kDa (predicted molecular weight: 234 kDa).
|
IP |
Use at 1-4 µg/mg of lysate.
|
Notes |
---|
WB
1/2000 - 1/10000. Detects a band of approximately >250 kDa (predicted molecular weight: 234 kDa). |
IP
Use at 1-4 µg/mg of lysate. |
Target
-
Relevance
SEC16A is required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. It defines endoplasmic reticulum exit sites (ERES). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. It is required for normal transitional endoplasmic reticulum (tER) organization. -
Cellular localization
Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Note=SAR1A activity is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. -
Database links
- Entrez Gene: 9919 Human
- Omim: 612854 Human
- SwissProt: O15027 Human
-
Alternative names
- FLJ26737 antibody
- KIAA0310 antibody
- OTTHUMP00000022589 antibody
see all
Images
-
All lanes : Anti-SEC16A/SEC16 antibody (ab70722) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 234 kDa
Observed band size: >250 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of Human SEC16A/SEC16 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70722 at 3 µg/mg lysate. Subsequent Western blot detection of SEC16A/SEC16 was performed using ab70722 at 0.04 µg/ml.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab70722 has been referenced in 3 publications.
- Corridoni D et al. NOD2 and TLR2 Signal via TBK1 and PI31 to Direct Cross-Presentation and CD8 T Cell Responses. Front Immunol 10:958 (2019). PubMed: 31114588
- Frattini V et al. A metabolic function of FGFR3-TACC3 gene fusions in cancer. Nature 553:222-227 (2018). PubMed: 29323298
- Willy JA et al. Function of inhibitor of Bruton's tyrosine kinase isoform a (IBTKa) in nonalcoholic steatohepatitis links autophagy and the unfolded protein response. J Biol Chem 292:14050-14065 (2017). PubMed: 28710282