Overview

  • Product name

  • Description

    Rabbit polyclonal to SEC22B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human SEC22B aa 100-150. The exact sequence is proprietary. (NP_004883.3)
    Database link: O75396

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as SEC22L1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab241585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 25 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus, cis-Golgi network membrane. Golgi apparatus, trans-Golgi network membrane. Melanosome. Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • ER-Golgi SNARE of 24 kDa antibody
    • ERS 24 antibody
    • ERS-24 antibody
    • ERS24 antibody
    • SC22B_HUMAN antibody
    • SEC22 vesicle trafficking protein homolog B antibody
    • SEC22 vesicle trafficking protein like 1 antibody
    • SEC22 vesicle-trafficking protein homolog B antibody
    • SEC22 vesicle-trafficking protein-like 1 antibody
    • Sec22b antibody
    • SEC22L 1 antibody
    • Vesicle trafficking protein SEC22b antibody
    • Vesicle-trafficking protein SEC22b antibody
    see all

Images

  • All lanes : Anti-SEC22B antibody (ab241585) at 1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 25 kDa


    Exposure time: 30 seconds


    Lysates prepared in NETN buffer.

  • SEC22B was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241585 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241585 at 1 µg/ml.

    Lane 1: ab241585 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in RIPA buffer.

References

ab241585 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241585.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up