• Product name

  • Description

    Rabbit polyclonal to SEC22B
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human SEC22B aa 100-150. The exact sequence is proprietary. (NP_004883.3)
    Database link: O75396

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as SEC22L1




Our Abpromise guarantee covers the use of ab241585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 25 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Function

    SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus, cis-Golgi network membrane. Golgi apparatus, trans-Golgi network membrane. Melanosome. Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • ER-Golgi SNARE of 24 kDa antibody
    • ERS 24 antibody
    • ERS-24 antibody
    • ERS24 antibody
    • SC22B_HUMAN antibody
    • SEC22 vesicle trafficking protein homolog B antibody
    • SEC22 vesicle trafficking protein like 1 antibody
    • SEC22 vesicle-trafficking protein homolog B antibody
    • SEC22 vesicle-trafficking protein-like 1 antibody
    • Sec22b antibody
    • SEC22L 1 antibody
    • Vesicle trafficking protein SEC22b antibody
    • Vesicle-trafficking protein SEC22b antibody
    see all


  • All lanes : Anti-SEC22B antibody (ab241585) at 1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 25 kDa

    Exposure time: 30 seconds

    Lysates prepared in NETN buffer.

  • SEC22B was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241585 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241585 at 1 µg/ml.

    Lane 1: ab241585 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in RIPA buffer.


ab241585 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab241585.
Please use the links above to contact us or submit feedback about this product.

For licensing inquiries, please contact partnerships@abcam.com

Sign up