Overview

  • Product name

    Anti-SEC22B antibody [EPR12335]
    See all SEC22B primary antibodies
  • Description

    Rabbit monoclonal [EPR12335] to SEC22B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SEC22B aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O75396

  • Positive control

    • MCF7 and SH-SY5Y cell lysates; Human breast tissue and ovarian carcinoma tissue; SH-SY5Y and MCF7 cells.
  • General notes

     

     

     This product was previously labelled as SEC22L1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/50 - 1/100.
IP 1/80.
Flow Cyt 1/110.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 longin domain.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus, cis-Golgi network membrane. Golgi apparatus, trans-Golgi network membrane. Melanosome. Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • ER-Golgi SNARE of 24 kDa antibody
    • ERS 24 antibody
    • ERS-24 antibody
    • ERS24 antibody
    • SC22B_HUMAN antibody
    • SEC22 vesicle trafficking protein homolog B antibody
    • SEC22 vesicle trafficking protein like 1 antibody
    • SEC22 vesicle-trafficking protein homolog B antibody
    • SEC22 vesicle-trafficking protein-like 1 antibody
    • Sec22b antibody
    • SEC22L 1 antibody
    • Vesicle trafficking protein SEC22b antibody
    • Vesicle-trafficking protein SEC22b antibody
    see all

Images

  • ab181076 (purified ) at 1/30 dilution (2ug) immunoprecipitating SEC22B in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab181076 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab181076 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP)�(ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling SEC22B with purified ab181076 at 1/50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SEC22B with purified ab181076 at 1/250 dilution (2.2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling SEC22B with purified ab181076 at 1/250 dilution (2.19 µg/ml). Heat mediated antigen retrieval using Bond� Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling SEC22B with purified ab181076 at 1/250 dilution (2.19 µg/ml). Heat mediated antigen retrieval using Bond� Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-SEC22B antibody [EPR12335] (ab181076) at 1/2000 dilution + SH-SY5Y cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa

  • Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling SEC22B with ab181076 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181076 at 1/80 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling SEC22B with ab181076 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SEC22B with ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SEC22B with ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Anti-SEC22B antibody [EPR12335] (ab181076) at 1/10000 dilution + MCF7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa

References

This product has been referenced in:

See 1 Publication for this product

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