Overview

  • Product name

  • Description

    Rabbit polyclonal to SEC23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 125-428 of Human SEC23 (Uniprot ID: Q15436)

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt-4 and Raji cells. Cal27 xenograft.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab137583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 86 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • Involvement in disease

    Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects.
  • Sequence similarities

    Belongs to the SEC23/SEC24 family. SEC23 subfamily.
  • Cellular localization

    Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLSD antibody
    • Protein transport protein Sec23A antibody
    • SC23A_HUMAN antibody
    • Sec23 homolog A (S. cerevisiae) antibody
    • SEC23-related protein A antibody
    • sec23a antibody
    see all

Images

  • Anti-SEC23 antibody (ab137583) at 1/10000 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size: 86 kDa



    7.5% SDS-PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labelling SEC23 with ab137583 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labelling SEC23 with ab137583 at 1/500 dilution.

References

This product has been referenced in:

  • Lassalle S  et al. MicroRNA-375/SEC23A as biomarkers of the in vitro efficacy of vandetanib. Oncotarget 7:30461-78 (2016). WB, IHC . Read more (PubMed: 27036030) »
See 1 Publication for this product

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There are currently no Customer reviews or Questions for ab137583.
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