Anti-SEC23A antibody (ab99552)
Key features and details
- Goat polyclonal to SEC23A
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SEC23A antibody
See all SEC23A primary antibodies -
Description
Goat polyclonal to SEC23A -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Horse, Cow, Dog, Human -
Immunogen
Synthetic peptide corresponding to Human SEC23A aa 335-346 (internal sequence).
Sequence:C-ANRAATTGHVID
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Positive control
- NIH 3T3 lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99552 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. -
Involvement in disease
Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects. -
Sequence similarities
Belongs to the SEC23/SEC24 family. SEC23 subfamily. -
Cellular localization
Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles. - Information by UniProt
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Database links
- Entrez Gene: 10484 Human
- Entrez Gene: 20334 Mouse
- Entrez Gene: 58817 Rat
- Omim: 610511 Human
- SwissProt: Q15436 Human
- SwissProt: Q01405 Mouse
- Unigene: 272927 Human
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Alternative names
- CLSD antibody
- Protein transport protein Sec23A antibody
- SC23A_HUMAN antibody
see all
Images
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Anti-SEC23A antibody (ab99552) at 2 µg/ml (incubation time 1 hour) + NIH 3T3 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab99552 has been referenced in 2 publications.
- Cao Q et al. Collagen VII maintains proteostasis in dermal fibroblasts by scaffolding TANGO1 cargo. Matrix Biol 111:226-244 (2022). PubMed: 35779741
- Dieterich IA et al. Acetyl-CoA flux from the cytosol to the ER regulates engagement and quality of the secretory pathway. Sci Rep 11:2013 (2021). PubMed: 33479349