Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SEC23 antibody [EPR13270(B)] (ab179811)

Overview

  • Product name

    Anti-SEC23 antibody [EPR13270(B)]
    See all SEC23 primary antibodies
  • Description

    Rabbit monoclonal [EPR13270(B)] to SEC23
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SEC23 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15436

  • Positive control

    • HeLa, HepG2 and 293T cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13270(B)
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab179811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 86 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
    • Involvement in disease

      Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects.
    • Sequence similarities

      Belongs to the SEC23/SEC24 family. SEC23 subfamily.
    • Cellular localization

      Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles.
    • Information by UniProt
    • Database links

    • Alternative names

      • CLSD antibody
      • Protein transport protein Sec23A antibody
      • SC23A_HUMAN antibody
      • Sec23 homolog A (S. cerevisiae) antibody
      • SEC23-related protein A antibody
      • sec23a antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SEC23 knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab179811 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab179811 was shown to specifically react with SEC23 when SEC23 knockout samples were used. Wild-type and SEC23 knockout samples were subjected to SDS-PAGE. ab179811 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-SEC23 antibody [EPR13270(B)] (ab179811) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 86 kDa

    • Flow Cytometrical analysis of permeabilized HeLa cells labeling SEC23 with ab179811 at 1/10 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Guillemyn B  et al. A homozygous pathogenic missense variant broadens the phenotypic and mutational spectrum of CREB3L1-related osteogenesis imperfecta. Hum Mol Genet N/A:N/A (2019). Read more (PubMed: 30657919) »
    • Keller RB  et al. Monoallelic and biallelic CREB3L1 variant causes mild and severe osteogenesis imperfecta, respectively. Genet Med 20:411-419 (2018). WB . Read more (PubMed: 28817112) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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