Key features and details
- Rabbit polyclonal to SEC23IP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SEC23IP antibody
See all SEC23IP primary antibodies
DescriptionRabbit polyclonal to SEC23IP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: RT4, U-251 MG sp, NIH/3T3 and NBT-II cell lysates. IHC-P: Human stomach tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 111 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionPlays a role in the organization of endoplasmic reticulum exit sites.
Tissue specificityUbiquitously expressed with stronger levels detected in heart, liver and skeletal muscle.
Sequence similaritiesBelongs to the PA-PLA1 family.
Contains 1 DDHD domain.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationCytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum.
- Information by UniProt
- MSTP053 antibody
- p125 antibody
- P125A antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SEC23IP (green) using ab224546 at 4 µg/ml in ICC/IF.
Paraffin-embedded human stomach tissue stained for SEC23IP using ab224546 at 1/20 dilution in immunohistochemical analysis.
All lanes : Anti-SEC23IP antibody (ab224546) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 111 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224546 has not yet been referenced specifically in any publications.