Overview

  • Product name

  • Description

    Rabbit polyclonal to SEC23IP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human SEC23IP aa 856-942.
    Sequence:

    HLELKESLSRMGSDLKQGFISSLKSAWQTLNEFARAHTSSTQLQEELEKV ANQIKEEEEKQVVEAEKVVESPDFSKDEDYLGKVGML


    Database link: Q9Y6Y8

  • Positive control

    • WB: RT4, U-251 MG sp, NIH/3T3 and NBT-II cell lysates. IHC-P: Human stomach tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab224546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 111 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilizatiom: PFA/Triton X-100.

Target

Images

  • All lanes : Anti-SEC23IP antibody (ab224546) at 1/100 dilution

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate

    Predicted band size: 111 kDa

  • PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SEC23IP (green) using ab224546 at 4 µg/ml in ICC/IF.

  • Paraffin-embedded human stomach tissue stained for SEC23IP using ab224546 at 1/20 dilution in immunohistochemical analysis.

  • All lanes : Anti-SEC23IP antibody (ab224546) at 1/100 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
    Lane 2 : NBT-II cell lysate

    Predicted band size: 111 kDa

References

ab224546 has not yet been referenced specifically in any publications.

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