Key features and details
- Rabbit polyclonal to SEC23IP
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SEC23IP antibody
See all SEC23IP primary antibodies
DescriptionRabbit polyclonal to SEC23IP
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 1 and 50 of human SEC23IP (NP_009121.1)
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70338 was affinity purified using an epitope specific to SEC23IP immobilized on solid support.
Our Abpromise guarantee covers the use of ab70338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2500 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 111 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a role in the organization of endoplasmic reticulum exit sites.
Tissue specificityUbiquitously expressed with stronger levels detected in heart, liver and skeletal muscle.
Sequence similaritiesBelongs to the PA-PLA1 family.
Contains 1 DDHD domain.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationCytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum.
- Information by UniProt
- MSTP053 antibody
- p125 antibody
- P125A antibody
All lanes : Anti-SEC23IP antibody (ab70338) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 111 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Detection of Human SEC23IP by Western Blot of Immunprecipitate. Lane 1: HeLa whole cell lysates immunoprecipitated using ab70338 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG used for immunoprecipitation. All lanes: ab70338 at 1µg/ml. Chemiluminescence with exposure times of 30 seconds
ab70338 has not yet been referenced specifically in any publications.