Anti-SEC23IP antibody (ab70338)
Key features and details
- Rabbit polyclonal to SEC23IP
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SEC23IP antibody
See all SEC23IP primary antibodies -
Description
Rabbit polyclonal to SEC23IP -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human SEC23IP (NP_009121.1)
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70338 was affinity purified using an epitope specific to SEC23IP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP | ||
WB | (1) |
Notes |
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WB: 1/2500 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 111 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Plays a role in the organization of endoplasmic reticulum exit sites. -
Tissue specificity
Ubiquitously expressed with stronger levels detected in heart, liver and skeletal muscle. -
Sequence similarities
Belongs to the PA-PLA1 family.
Contains 1 DDHD domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Cytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 11196 Human
- SwissProt: Q9Y6Y8 Human
- Unigene: 435004 Human
- Unigene: 667094 Human
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Alternative names
- MSTP053 antibody
- p125 antibody
- P125A antibody
see all
Images
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All lanes : Anti-SEC23IP antibody (ab70338) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 111 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of Human SEC23IP by Western Blot of Immunprecipitate. Lane 1: HeLa whole cell lysates immunoprecipitated using ab70338 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG used for immunoprecipitation. All lanes: ab70338 at 1µg/ml. Chemiluminescence with exposure times of 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab70338 has been referenced in 1 publication.
- Aghazadeh Y et al. Identification of Sec23ip, Part of 14-3-3? Protein Network, as a Regulator of Acute Steroidogenesis in MA-10 Leydig Cells. Endocrinology 161:N/A (2020). PubMed: 31875919