Key features and details
- Rabbit polyclonal to SEC23IP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SEC23IP antibody
See all SEC23IP primary antibodies
DescriptionRabbit polyclonal to SEC23IP
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to a region between residues 350 and 400 of human SEC23IP (NP_009121.1).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70339 was affinity purified using an epitope specific to SEC23IP immobilized on solid support.
Our Abpromise guarantee covers the use of ab70339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2500 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 111 kDa).|
FunctionPlays a role in the organization of endoplasmic reticulum exit sites.
Tissue specificityUbiquitously expressed with stronger levels detected in heart, liver and skeletal muscle.
Sequence similaritiesBelongs to the PA-PLA1 family.
Contains 1 DDHD domain.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationCytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum.
- Information by UniProt
- MSTP053 antibody
- p125 antibody
- P125A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SEC23IP with ab70339 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-SEC23IP antibody (ab70339) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 111 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
ab70339 has not yet been referenced specifically in any publications.