Overview

  • Product name

  • Description

    Rabbit polyclonal to Sec24C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: RLPSMTGPLL PGQSFGGPSV SQPNHVSSPP QALPPGTQMT GPLGPLPPMH SPQQPGYQPQ QNGSFGPARG PQSNYGGPYP AAPTF, corresponding to internal sequence amino acids 222-306 of Human Sec24C.

  • Positive control

    • U 251 MG cells; RT 4, U 251 MG, Human liver and tonsil lysates; Human stomach tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
WB 1/250 - 1/500.

Target

  • Function

    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the SEC23/SEC24 family. SEC24 subfamily.
  • Cellular localization

    Cytoplasm. Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein transport protein Sec24C antibody
    • SC 24C antibody
    • SC24 C antibody
    • SC24C antibody
    • SC24C_HUMAN antibody
    • Sec 24C antibody
    • Sec24 C antibody
    • SEC24 related gene family, member C (S. cerevisiae) antibody
    • SEC24 related protein C antibody
    • SEC24-related protein C antibody
    • SEC24C antibody
    see all

Images

  • All lanes : Anti-Sec24C antibody (ab122633) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique.
  • ab122633 at 1/200 dilution staining Sec24C in paraffin-embedded Human stomach tissue by Immunohistochemistry.
  • ab122633 at 4 µg/ml staining SEC24C in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

References

This product has been referenced in:

See 1 Publication for this product

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