RecombinantRabMAb

Recombinant Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)

Reviews (2) Submit a question References (4)
Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)
  • Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)
  • Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16089] to SEC24D - N-terminal
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SEC24D antibody [EPR16089] - N-terminal
    See all SEC24D primary antibodies

  • Description

    Rabbit monoclonal [EPR16089] to SEC24D - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SEC24D aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O94855

  • Positive control

    • SH-SY5Y, HeLa, Raw264.7, PC-12 and NIH/3T3 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab191566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

Target

  • Function

    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.

  • Tissue specificity

    Ubiquitously expressed, with higher amounts in placenta, pancreas, heart and liver.

  • Sequence similarities

    Belongs to the SEC23/SEC24 family. SEC24 subfamily.

  • Cellular localization

    Cytoplasm. Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • KIAA0755 antibody
      • Protein transport protein Sec24D antibody
      • SC24D_HUMAN antibody
      • SEC24 family member D antibody
      • SEC24 related gene family, member D antibody
      • SEC24-related protein D antibody
      • SEC24D antibody
      see all

    Images

    • Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)
      Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)
      All lanes : Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566) at 1/5000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 113 kDa
      Observed band size: 113 kDa



      Blocking/Dilution buffer: 5% NFDM /TBST.

    • Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)
      Western blot - Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)
      All lanes : Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566) at 1/1000 dilution

      Lane 1 : Raw264.7 cell lysate
      Lane 2 : PC-12 cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 113 kDa
      Observed band size: 113 kDa



      Blocking/Dilution buffer: 5% NFDM /TBST.

    • Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)
      Anti-SEC24D antibody [EPR16089] - N-terminal (ab191566)

    References (4)

    Publishing research using ab191566? Please let us know so that we can cite the reference in this datasheet.

    ab191566 has been referenced in 4 publications.

    • Viviano J  et al. ERp29 as a regulator of Insulin biosynthesis. PLoS One 15:e0233502 (2020). PubMed: 32433667
    • Guillemyn B  et al. A homozygous pathogenic missense variant broadens the phenotypic and mutational spectrum of CREB3L1-related osteogenesis imperfecta. Hum Mol Genet N/A:N/A (2019). PubMed: 30657919
    • Keller RB  et al. Monoallelic and biallelic CREB3L1 variant causes mild and severe osteogenesis imperfecta, respectively. Genet Med 20:411-419 (2018). WB . PubMed: 28817112
    • Fan J  et al. cTAGE5 deletion in pancreatic ß cells impairs proinsulin trafficking and insulin biogenesis in mice. J Cell Biol 216:4153-4164 (2017). PubMed: 29133483

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-2 of 2 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SEC24D antibody [EPR16089] - N-terminal

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - 0.5% triton-X100
    Specification
    U2OS
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 17 2020

    Western blot abreview for Anti-SEC24D antibody [EPR16089] - N-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    293T
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More

    Dr. Jerome Cattin

    Verified customer

    Submitted May 20 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com