Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - 0.5% triton-X100
Specification
U2OS
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 20% FCS, 1% tween-20 in PBS
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: goat anti-rabbit alexa fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Abcam response
Abcam has not tested ab191566 in ICC/IF and thus can't say how it should have performed.
Abcam user community
Verified customer
Submitted Jun 17 2020