Product nameAnti-Sec31A antibody
See all Sec31A primary antibodies
DescriptionRabbit polyclonal to Sec31A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Pig, Chimpanzee, Ferret, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 775 and 825 of human Sec31A (NP_001070675.1)
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceThe protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function.
Cellular localizationCytoplasm. Cytoplasmic vesicle; COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Note: Associates with membranes in a GTP-dependent manner
- 1810024J13Rik antibody
- ABP125 antibody
- ABP130 antibody
All lanes : Anti-Sec31A antibody (ab86600) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 133 kDa
Observed band size: 133 kDa
Exposure time: 3 seconds
Detection of Sec31A by Western Blot of Immunprecipitate.
ab86600 at 0.1µg/ml staining Sec31A in HeLa whole cell lysate immunoprecipitated using ab86600 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
ab86600 has not yet been referenced specifically in any publications.