Anti-SEC61A antibody (ab1327)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-SEC61A antibody
See all SEC61A primary antibodies -
DescriptionGoat polyclonal to SEC61A
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Host speciesGoat
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Dog, Pig
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Immunogen
Synthetic peptide:
C-KEQSEVGSMGALLF
, corresponding to C terminal amino acids 463-476 of Human SEC61A(Peptide available as ab22804.) -
Positive control
- Human cerebellum and mouse brain lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab1327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human SEC61A peptide (ab22804). | |
| IHC-P | Use a concentration of 3.75 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 19934005 |
Target
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FunctionPlays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
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Sequence similaritiesBelongs to the secY/SEC61-alpha family.
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Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
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Database links
- Entrez Gene: 505064 Cow
- Entrez Gene: 404006 Dog
- Entrez Gene: 29927 Human
- Entrez Gene: 53421 Mouse
- Omim: 609213 Human
- SwissProt: Q5EA68 Cow
- SwissProt: P38377 Dog
- SwissProt: P61619 Human
see all -
Alternative names
- chunp6898 antibody
- fb62c11 antibody
- HSEC61 antibody
see all
Images
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Western blot - Anti-SEC61A antibody (ab1327)Anti-SEC61A antibody (ab1327) at 1 µg/ml + Human cerebellum lysate in RIPA buffer at 35 µg
Predicted band size: 52 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Lloyd DJ et al. A point mutation in Sec61alpha1 leads to diabetes and hepatosteatosis in mice. Diabetes 59:460-70 (2010). ICC/IF ; Mouse . Read more (PubMed: 19934005) »
