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    sec61a-antibody-ab1327.pdf

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Signal Transduction Protein Trafficking ER Proteins
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Anti-SEC61A antibody (ab1327)

  • Datasheet
  • SDS
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Western blot - Anti-SEC61A antibody (ab1327)

    Key features and details

    • Goat polyclonal to SEC61A
    • Suitable for: WB, IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

    View more associated products

    Overview

    • Product name

      Anti-SEC61A antibody
      See all SEC61A primary antibodies
    • Description

      Goat polyclonal to SEC61A
    • Host species

      Goat
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      C-KEQSEVGSMGALLF

      , corresponding to C terminal amino acids 463-476 of Human SEC61A(Peptide available as ab22804.)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.05% Tris, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • ER Proteins

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1327 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Cow
    Dog
    Pig
    Application Abreviews Notes
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

    1 hour primary incubation is recommended for this product.

    IHC-P
    Use a concentration of 3.75 µg/ml.
    Notes
    WB
    Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).

    1 hour primary incubation is recommended for this product.

    IHC-P
    Use a concentration of 3.75 µg/ml.

    Target

    • Function

      Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
    • Sequence similarities

      Belongs to the secY/SEC61-alpha family.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession P61619 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 505064 Cow
      • Entrez Gene: 404006 Dog
      • Entrez Gene: 29927 Human
      • Omim: 609213 Human
      • SwissProt: Q5EA68 Cow
      • SwissProt: P38377 Dog
      • SwissProt: P61619 Human
      • Unigene: 518236 Human
      • Alternative names

        • chunp6898 antibody
        • fb62c11 antibody
        • HSEC61 antibody
        • protein transport protein SEC61 alpha subunit antibody
        • protein transport protein Sec61 subunit alpha antibody
        • Protein transport protein Sec61 subunit alpha isoform 1 antibody
        • S61A1_HUMAN antibody
        • SEC 61 antibody
        • SEC 61A antibody
        • Sec61 alpha 1 antibody
        • Sec61 alpha 1 subunit antibody
        • Sec61 alpha 1 subunit (S. cerevisiae) antibody
        • Sec61 alpha-1 antibody
        • SEC61 antibody
        • Sec61 homolog antibody
        • SEC61A1 antibody
        • sec61aa antibody
        see all

      Images

      • Western blot - Anti-SEC61A antibody (ab1327)
        Western blot - Anti-SEC61A antibody (ab1327)
        Anti-SEC61A antibody (ab1327) at 1 µg/ml + Human cerebellum lysate in RIPA buffer at 35 µg

        Predicted band size: 52 kDa

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab1327? Please let us know so that we can cite the reference in this datasheet.

      ab1327 has been referenced in 1 publication.

      • Lloyd DJ  et al. A point mutation in Sec61alpha1 leads to diabetes and hepatosteatosis in mice. Diabetes 59:460-70 (2010). ICC/IF ; Mouse . PubMed: 19934005

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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