Overview

  • Product name
    Anti-SEC61A antibody
    See all SEC61A primary antibodies
  • Description
    Goat polyclonal to SEC61A
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-KEQSEVGSMGALLF

    , corresponding to C terminal amino acids 463-476 of Human SEC61A(Peptide available as ab22804.)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human cerebellum and mouse brain lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human SEC61A peptide (ab22804).
IHC-P Use a concentration of 3.75 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 19934005

Target

  • Function
    Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
  • Sequence similarities
    Belongs to the secY/SEC61-alpha family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • chunp6898 antibody
    • fb62c11 antibody
    • HSEC61 antibody
    • protein transport protein SEC61 alpha subunit antibody
    • protein transport protein Sec61 subunit alpha antibody
    • Protein transport protein Sec61 subunit alpha isoform 1 antibody
    • S61A1_HUMAN antibody
    • SEC 61 antibody
    • SEC 61A antibody
    • Sec61 alpha 1 antibody
    • Sec61 alpha 1 subunit antibody
    • Sec61 alpha 1 subunit (S. cerevisiae) antibody
    • Sec61 alpha-1 antibody
    • SEC61 antibody
    • Sec61 homolog antibody
    • SEC61A1 antibody
    • sec61aa antibody
    see all

Images

  • Western blot - Anti-SEC61A antibody (ab1327)
    Western blot - Anti-SEC61A antibody (ab1327)
    Anti-SEC61A antibody (ab1327) at 1 µg/ml + Human cerebellum lysate in RIPA buffer at 35 µg

    Predicted band size: 52 kDa

References

This product has been referenced in:
  • Lloyd DJ  et al. A point mutation in Sec61alpha1 leads to diabetes and hepatosteatosis in mice. Diabetes 59:460-70 (2010). ICC/IF ; Mouse . Read more (PubMed: 19934005) »

See 1 Publication for this product

Publishing research using ab1327? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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