Anti-SEC61A antibody (ab1327)
Key features and details
- Goat polyclonal to SEC61A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SEC61A antibody
See all SEC61A primary antibodies -
Description
Goat polyclonal to SEC61A -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
C-KEQSEVGSMGALLF
, corresponding to C terminal amino acids 463-476 of Human SEC61A(Peptide available as ab22804.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Cow
Dog
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 3.75 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins. -
Sequence similarities
Belongs to the secY/SEC61-alpha family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 505064 Cow
- Entrez Gene: 404006 Dog
- Entrez Gene: 29927 Human
- Omim: 609213 Human
- SwissProt: Q5EA68 Cow
- SwissProt: P38377 Dog
- SwissProt: P61619 Human
- Unigene: 518236 Human
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Alternative names
- chunp6898 antibody
- fb62c11 antibody
- HSEC61 antibody
see all
Images
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Western blot - Anti-SEC61A antibody (ab1327)Anti-SEC61A antibody (ab1327) at 1 µg/ml + Human cerebellum lysate in RIPA buffer at 35 µg
Predicted band size: 52 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab1327 has been referenced in 1 publication.
- Lloyd DJ et al. A point mutation in Sec61alpha1 leads to diabetes and hepatosteatosis in mice. Diabetes 59:460-70 (2010). ICC/IF ; Mouse . PubMed: 19934005