Anti-SEC61A antibody (ab1327)
Key features and details
- Goat polyclonal to SEC61A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SEC61A antibody
See all SEC61A primary antibodies -
Description
Goat polyclonal to SEC61A -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig -
Immunogen
Synthetic peptide:
C-KEQSEVGSMGALLF
, corresponding to C terminal amino acids 463-476 of Human SEC61A(Peptide available as ab22804.) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 3.75 µg/ml.
|
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins. -
Sequence similarities
Belongs to the secY/SEC61-alpha family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 505064 Cow
- Entrez Gene: 404006 Dog
- Entrez Gene: 29927 Human
- Omim: 609213 Human
- SwissProt: Q5EA68 Cow
- SwissProt: P38377 Dog
- SwissProt: P61619 Human
- Unigene: 518236 Human
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Alternative names
- chunp6898 antibody
- fb62c11 antibody
- HSEC61 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab1327 has been referenced in 1 publication.
- Lloyd DJ et al. A point mutation in Sec61alpha1 leads to diabetes and hepatosteatosis in mice. Diabetes 59:460-70 (2010). ICC/IF ; Mouse . PubMed: 19934005