Overview

  • Product name

    Anti-SEC61A antibody [EPR14379]
    See all SEC61A primary antibodies
  • Description

    Rabbit monoclonal [EPR14379] to SEC61A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SEC61A aa 350-450. The exact sequence is proprietary.
    Database link: Q9H9S3

  • Positive control

    • Human fetal brain, Daudi and A431 lysates; Human Infiltrating duct carcinoma of breast tissue; A431 and Daudi cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14379
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40 - 1/60.
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
  • Sequence similarities

    Belongs to the secY/SEC61-alpha family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • chunp6898 antibody
    • fb62c11 antibody
    • HSEC61 antibody
    • protein transport protein SEC61 alpha subunit antibody
    • protein transport protein Sec61 subunit alpha antibody
    • Protein transport protein Sec61 subunit alpha isoform 1 antibody
    • S61A1_HUMAN antibody
    • SEC 61 antibody
    • SEC 61A antibody
    • Sec61 alpha 1 antibody
    • Sec61 alpha 1 subunit antibody
    • Sec61 alpha 1 subunit (S. cerevisiae) antibody
    • Sec61 alpha-1 antibody
    • SEC61 antibody
    • Sec61 homolog antibody
    • SEC61A1 antibody
    • sec61aa antibody
    see all

Images

  • All lanes : Anti-SEC61A antibody [EPR14379] (ab183046) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 52 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?

  • Western blot analysis of SEC61A in Daudi cell lysate immunoprecipitated using ab183046 at 1/50 dilution (Lane 1). Lane 2: Negative control.

    Secondary antibody: Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1500 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Daudi cells labeling SEC61A with ab183046 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 diution.

  • Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling SEC61A with ab183046 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

This product has been referenced in:

  • Puri C  et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell 45:114-131.e8 (2018). WB . Read more (PubMed: 29634932) »
  • Tian S  et al. Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation. PLoS Biol 16:e2006951 (2018). Read more (PubMed: 30481169) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain section)
Permeabilization
Yes - 0.4% Triton
Specification
brain section
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 03 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up