Key features and details
- Rabbit polyclonal to SEC61B
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SEC61B antibody
See all SEC61B primary antibodies
DescriptionRabbit polyclonal to SEC61B
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Dog, Orangutan
- IHC-P: Human pancreas, liver, cerebellum and duodenum tissue. ICC/IF: PC-3 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionNecessary for protein translocation in the endoplasmic reticulum.
Sequence similaritiesBelongs to the SEC61-beta family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Protein translocation complex beta antibody
- Protein transport protein SEC61 beta subunit antibody
- Protein transport protein Sec61 subunit beta antibody
Paraffin-embedded human duodenum tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for SEC61B (green) using ab244487 at 4 μg/ml in ICC/IF.
Paraffin-embedded human cerebellum tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for SEC61B using ab244487 at 1/200 dilution in immunohistochemical analysis. No positivity in myocytes, as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244487 has not yet been referenced specifically in any publications.