Anti-SEC61B antibody - N-terminal (ab229542)

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Overview

  • Product name

    Anti-SEC61B antibody - N-terminal
    See all SEC61B primary antibodies

  • Description

    Rabbit polyclonal to SEC61B - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human SEC61B (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
    Database link: P60468

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse liver extract. IP: HepG2 whole cell extract.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 10 kDa.
IP 1/100 - 1/500.

Target

Images

  • Western blot - Anti-SEC61B antibody - N-terminal (ab229542)
    Western blot - Anti-SEC61B antibody - N-terminal (ab229542)
    All lanes : Anti-SEC61B antibody - N-terminal (ab229542) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 10 kDa



    15% SDS-PAGE gel.

  • Western blot - Anti-SEC61B antibody - N-terminal (ab229542)
    Western blot - Anti-SEC61B antibody - N-terminal (ab229542)
    Anti-SEC61B antibody - N-terminal (ab229542) at 1/1000 dilution + Mouse liver extract at 50 µg

    Predicted band size: 10 kDa



    15% SDS-PAGE gel.

  • Immunoprecipitation - Anti-SEC61B antibody - N-terminal (ab229542)
    Immunoprecipitation - Anti-SEC61B antibody - N-terminal (ab229542)

    SEC61B was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab229542. Western blot was performed from the immunoprecipitate using ab229542. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: HepG2 whole cell extract (Input). 

    Lane 2: Control IgG instead of ab229542 in HepG2 whole cell extract. 

    Lane 3: ab229542 IP in HepG2 whole cell extract. 

References

ab229542 has not yet been referenced specifically in any publications.

Publishing research using ab229542? Please let us know so that we can cite the reference in this datasheet.

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