Anti-SEC61B antibody - N-terminal (ab229542)
Key features and details
- Rabbit polyclonal to SEC61B - N-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SEC61B antibody - N-terminal
See all SEC61B primary antibodies -
Description
Rabbit polyclonal to SEC61B - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SEC61B (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P60468 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse liver extract. IP: HepG2 whole cell extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab229542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 10 kDa. | |
| IP | 1/100 - 1/500. |
Target
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Function
Necessary for protein translocation in the endoplasmic reticulum. -
Sequence similarities
Belongs to the SEC61-beta family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 10952 Human
- Entrez Gene: 66212 Mouse
- Omim: 609214 Human
- SwissProt: P60468 Human
- SwissProt: Q9CQS8 Mouse
- Unigene: 191887 Human
- Unigene: 20188 Mouse
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Alternative names
- Protein translocation complex beta antibody
- Protein transport protein SEC61 beta subunit antibody
- Protein transport protein Sec61 subunit beta antibody
see all
Images
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Western blot - Anti-SEC61B antibody - N-terminal (ab229542)All lanes : Anti-SEC61B antibody - N-terminal (ab229542) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 10 kDa15% SDS-PAGE gel.
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Western blot - Anti-SEC61B antibody - N-terminal (ab229542)Anti-SEC61B antibody - N-terminal (ab229542) at 1/1000 dilution + Mouse liver extract at 50 µg
Predicted band size: 10 kDa15% SDS-PAGE gel.
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Immunoprecipitation - Anti-SEC61B antibody - N-terminal (ab229542)SEC61B was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg of ab229542. Western blot was performed from the immunoprecipitate using ab229542. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HepG2 whole cell extract (Input).
Lane 2: Control IgG instead of ab229542 in HepG2 whole cell extract.
Lane 3: ab229542 IP in HepG2 whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229542 has not yet been referenced specifically in any publications.
