Key features and details
- Rabbit polyclonal to SEC62
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SEC62 antibody
See all SEC62 primary antibodies
DescriptionRabbit polyclonal to SEC62
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab168843 was affinity purified using an epitope specific to SEC62 immobilized on solid support.
Our Abpromise guarantee covers the use of ab168843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 46 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRequired for preprotein translocation.
Sequence similaritiesBelongs to the SEC62 family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Dtrp1 antibody
- FLJ32803 antibody
- hTP-1 antibody
All lanes : Anti-SEC62 antibody (ab168843) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 10 seconds
Detection of SEC62 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168843 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab168843 has been referenced in 1 publication.
- OhAinle M et al. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV. Elife 7:N/A (2018). PubMed: 30520725