Recombinant Anti-SEC62 antibody [EPR9212] (ab137022)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9212] to SEC62
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SEC62 antibody [EPR9212]
See all SEC62 primary antibodies -
Description
Rabbit monoclonal [EPR9212] to SEC62 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SEC62 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, K562, HepG2, and BxPC-3 cell lysates; Human brain and placenta tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9212 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
IHC-P
1/100 - 1/250. |
Target
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Function
Required for preprotein translocation. -
Sequence similarities
Belongs to the SEC62 family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 7095 Human
- Entrez Gene: 69276 Mouse
- Entrez Gene: 294912 Rat
- Omim: 602173 Human
- SwissProt: Q99442 Human
- SwissProt: Q8BU14 Mouse
- Unigene: 592561 Human
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Alternative names
- Dtrp1 antibody
- FLJ32803 antibody
- hTP-1 antibody
see all
Images
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All lanes : Anti-SEC62 antibody [EPR9212] (ab137022) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling SEC62 with ab137022 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling SEC62 with ab137022 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab137022 has been referenced in 3 publications.
- Chipurupalli S et al. Cancer cells adapt FAM134B/BiP mediated ER-phagy to survive hypoxic stress. Cell Death Dis 13:357 (2022). PubMed: 35436985
- Du J et al. Sec62 promotes early recurrence of hepatocellular carcinoma through activating integrina/CAV1 signalling. Oncogenesis 8:74 (2019). PubMed: 31822656
- Cunningham CN et al. Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates. Mol Cell 75:442-456.e4 (2019). PubMed: 31176671