Overview

  • Product name

    Anti-SEC62 antibody [EPR9213]
    See all SEC62 primary antibodies
  • Description

    Rabbit monoclonal [EPR9213] to SEC62
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SEC62 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • HeLa, SH-SY5Y, HepG2, NIH/3T3 and C6 cell lysates. Human kidney tissue and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • All lanes : Anti-SEC62 antibody [EPR9213] (ab140644) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : HepG2 cell lysates
      Lane 4 : NIH/3T3 cell lysates
      Lane 5 : C6 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size: 46 kDa

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labelling SEC62 with ab140644 at a 1/250 dilution.
    • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling SEC62 with ab140644 at a 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140644 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140644 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140644 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab140644 showing +ve staining.

    References

    ab140644 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab140644.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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