Product nameAnti-Sec8 antibody
See all Sec8 primary antibodies
DescriptionRabbit polyclonal to Sec8
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: RT4 and U-251 MG sp cell lysates. IHC-P: Human duodenum tissue. ICC/IF: U-251 MG cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/250. Predicted molecular weight: 110 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionComponent of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Sequence similaritiesBelongs to the SEC8 family.
- Information by UniProt
- dSec8 antibody
- EXOC 4 antibody
- Exoc4 antibody
All lanes : Anti-Sec8 antibody (ab224484) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG sp (human brain glioma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 110 kDa
Paraffin-embedded human duodenum tissue stained for Sec8 using ab224484 at 1/50 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Sec8 (green) using ab224484 at 4 µg/ml in ICC/IF.
ab224484 has not yet been referenced specifically in any publications.