Anti-Sec8 antibody - C-terminal (ab205945)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Sec8 antibody - C-terminal
See all Sec8 primary antibodies -
Description
Rabbit polyclonal to Sec8 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human Sec8 aa 924-974 (C terminal). The exact sequence is proprietary.
Sequence:EYIHALT LLHRSQTGVG ELTTQNTRLQ RLKEIICEQA AIKQATKDKK ITTV
Database link: Q96A65 -
Positive control
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab205945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 110 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. -
Sequence similarities
Belongs to the SEC8 family. - Information by UniProt
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Database links
- Entrez Gene: 60412 Human
- Entrez Gene: 20336 Mouse
- Entrez Gene: 116654 Rat
- Omim: 608185 Human
- SwissProt: Q96A65 Human
- SwissProt: O35382 Mouse
- SwissProt: Q62824 Rat
- Unigene: 321273 Human
see all -
Alternative names
- dSec8 antibody
- EXOC 4 antibody
- Exoc4 antibody
see all
Images
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Immunoprecipitation - Anti-Sec8 antibody - C-terminal (ab205945)Lane 1: Detection of Human Sec8 by Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab205945 at 6 µg for IP.
Lane 2: Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab205945 at 1 µg/ml. -
Western blot - Anti-Sec8 antibody - C-terminal (ab205945)All lanes : Anti-Sec8 antibody - C-terminal (ab205945) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells
Lane 2 : Whole cell lysate from 293T cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from mouse TCMK-1 cells
Lane 5 : Whole cell lysate from mouse NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 30 seconds
Datasheets and documents
References
ab205945 has not yet been referenced specifically in any publications.
