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  1. Link

    sec8-antibody-c-terminal-ab205945.pdf

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Anti-Sec8 antibody - C-terminal (ab205945)

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  • Immunoprecipitation - Anti-Sec8 antibody - C-terminal (ab205945)
  • Western blot - Anti-Sec8 antibody - C-terminal (ab205945)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-Sec8 antibody - C-terminal
    See all Sec8 primary antibodies
  • Description

    Rabbit polyclonal to Sec8 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Sec8 aa 924-974 (C terminal). The exact sequence is proprietary.
    Sequence:

    EYIHALT LLHRSQTGVG ELTTQNTRLQ RLKEIICEQA AIKQATKDKK ITTV


    Database link: Q96A65

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
    • 293T whole cell lysate (ab95494)
  • Recombinant Protein

    • Recombinant Human Sec8 protein (ab153474)

Applications

Our Abpromise guarantee covers the use of ab205945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 110 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
  • Sequence similarities

    Belongs to the SEC8 family.
  • Target information above from: UniProt accession Q96A65 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 60412 Human
    • Entrez Gene: 20336 Mouse
    • Entrez Gene: 116654 Rat
    • Omim: 608185 Human
    • SwissProt: Q96A65 Human
    • SwissProt: O35382 Mouse
    • SwissProt: Q62824 Rat
    • Unigene: 321273 Human
    • Unigene: 265512 Mouse
    • Unigene: 51841 Rat
    see all
  • Alternative names

    • dSec8 antibody
    • EXOC 4 antibody
    • Exoc4 antibody
    • EXOC4_HUMAN antibody
    • Exocyst complex component 4 antibody
    • Exocyst complex component SEC8 antibody
    • KIAA1699 antibody
    • MGC27170 antibody
    • REC 8 antibody
    • REC8 antibody
    • rSec 8 antibody
    • Sec 8 antibody
    • Sec 8p antibody
    • Sec8 antibody
    • SEC8 like 1 antibody
    • SEC8 protein antibody
    • Sec8L1 antibody
    • Sec8p antibody
    see all

Images

  • Immunoprecipitation - Anti-Sec8 antibody - C-terminal (ab205945)
    Immunoprecipitation - Anti-Sec8 antibody - C-terminal (ab205945)

    Lane 1: Detection of Human Sec8 by Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab205945 at 6 µg for IP.
    Lane 2: Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using Control IgG.
    Subsequent Western blot detection used ab205945 at 1 µg/ml.

  • Western blot - Anti-Sec8 antibody - C-terminal (ab205945)
    Western blot - Anti-Sec8 antibody - C-terminal (ab205945)
    All lanes : Anti-Sec8 antibody - C-terminal (ab205945) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells
    Lane 2 : Whole cell lysate from 293T cells
    Lane 3 : Whole cell lysate from Jurkat cells
    Lane 4 : Whole cell lysate from mouse TCMK-1 cells
    Lane 5 : Whole cell lysate from mouse NIH3T3 cells

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 110 kDa


    Exposure time: 30 seconds

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Datasheets and documents

    • Datasheet
  • References

    ab205945 has not yet been referenced specifically in any publications.

    Publishing research using ab205945? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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