Anti-Sec8 antibody - C-terminal (ab205945)
Key features and details
- Rabbit polyclonal to Sec8 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Sec8 antibody - C-terminal
See all Sec8 primary antibodies -
Description
Rabbit polyclonal to Sec8 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide within Human Sec8 aa 924-974 (C terminal). The exact sequence is proprietary.
Sequence:EYIHALT LLHRSQTGVG ELTTQNTRLQ RLKEIICEQA AIKQATKDKK ITTV
Database link: Q96A65 -
Positive control
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab205945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 110 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. -
Sequence similarities
Belongs to the SEC8 family. - Information by UniProt
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Database links
- Entrez Gene: 60412 Human
- Entrez Gene: 20336 Mouse
- Entrez Gene: 116654 Rat
- Omim: 608185 Human
- SwissProt: Q96A65 Human
- SwissProt: O35382 Mouse
- SwissProt: Q62824 Rat
- Unigene: 321273 Human
see all -
Alternative names
- dSec8 antibody
- EXOC 4 antibody
- Exoc4 antibody
see all
Images
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Immunoprecipitation - Anti-Sec8 antibody - C-terminal (ab205945)Lane 1: Detection of Human Sec8 by Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab205945 at 6 µg for IP.
Lane 2: Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab205945 at 1 µg/ml. -
Western blot - Anti-Sec8 antibody - C-terminal (ab205945)All lanes : Anti-Sec8 antibody - C-terminal (ab205945) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells
Lane 2 : Whole cell lysate from 293T cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from mouse TCMK-1 cells
Lane 5 : Whole cell lysate from mouse NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
References (0)
ab205945 has not yet been referenced specifically in any publications.
