Product nameAnti-Sec8 antibody - C-terminal
See all Sec8 primary antibodies
DescriptionRabbit polyclonal to Sec8 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab205945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 110 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionComponent of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.
Sequence similaritiesBelongs to the SEC8 family.
- Information by UniProt
- dSec8 antibody
- EXOC 4 antibody
- Exoc4 antibody
Lane 1: Detection of Human Sec8 by Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using ab205945 at 6 µg for IP.
Lane 2: Immunoprecipitation from Jurkat whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab205945 at 1 µg/ml.
All lanes : Anti-Sec8 antibody - C-terminal (ab205945) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells
Lane 2 : Whole cell lysate from 293T cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from mouse TCMK-1 cells
Lane 5 : Whole cell lysate from mouse NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 30 seconds
ab205945 has not yet been referenced specifically in any publications.