Key features and details
- Mouse monoclonal [2957C2a] to SECISBP2
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SECISBP2 antibody [2957C2a]
See all SECISBP2 primary antibodies
DescriptionMouse monoclonal [2957C2a] to SECISBP2
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment of human SECISBP2 derived from a near N terminal internal sequence.
- HEK293 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityProtein G purified
Purification notesab70601 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab70601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).|
|Dot blot||Use at an assay dependent dilution.|
FunctionBinds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB.
Tissue specificityExpressed at high levels in testis.
Involvement in diseaseDefects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM) [MIM:609698]. This phenotype is associated with a reduction in type II iodothyronine deiodinase activity.
- Information by UniProt
- DKFZp686C09169 antibody
- OTTHUMP00000064929 antibody
- OTTHUMP00000064930 antibody
Anti-SECISBP2 antibody [2957C2a] (ab70601) at 2 µg/ml + HEK293 whole cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
ab70601 has not yet been referenced specifically in any publications.