Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab53534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 3 µg/ml.|
FunctionBinds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB.
Tissue specificityExpressed at high levels in testis.
Involvement in diseaseDefects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM) [MIM:609698]. This phenotype is associated with a reduction in type II iodothyronine deiodinase activity.
- Information by UniProt
- DKFZp686C09169 antibody
- OTTHUMP00000064929 antibody
- OTTHUMP00000064930 antibody
All lanes : Anti-SECISBP2 antibody (ab53534) at 1/5000 dilution
Lanes 1-2 : Mouse testis lysate - cytosolic fraction (50µg)
Lane 3 : Mouse liver lysate - cytosolic fraction (50µg)
Lane 4 : Mouse kidney lysate - cytosolic fraction (50µg)
Lanes 5-6 : Mouse brain lysate - cytosolic fraction (50µg)
Lane 7 : Human HepG2 Lysate - cytosolic fraction (50µg)
Lane 8 : Rat testis lysate - cytosolic fraction (50µg)
All lanes : Donkey anti-goat conjugated to HRP
Predicted band size: 95 kDa
Bar indicates SECISBP2.
ab53534 at 3ug/ml staining SECISBP2 in human testis tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). The tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.
ab53534 has not yet been referenced specifically in any publications.