Anti-SECISBP2 antibody (ab53534)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-SECISBP2 antibody
See all SECISBP2 primary antibodies -
Description
Goat polyclonal to SECISBP2 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IF, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig
-
Immunogen
Synthetic peptide:
C-KERQERKQRLQEN
, corresponding to internal sequence amino acids 538-550 of Human SECISBP2
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab53534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 3 µg/ml. |
Target
-
Function
Binds to the SECIS element in the 3'-UTR of some mRNAs encoding selenoproteins. Binding is stimulated by SELB. -
Tissue specificity
Expressed at high levels in testis. -
Involvement in disease
Defects in SECISBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM) [MIM:609698]. This phenotype is associated with a reduction in type II iodothyronine deiodinase activity. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 511202 Cow
- Entrez Gene: 476351 Dog
- Entrez Gene: 79048 Human
- Omim: 607693 Human
- SwissProt: Q96T21 Human
- Unigene: 59804 Human
-
Alternative names
- DKFZp686C09169 antibody
- OTTHUMP00000064929 antibody
- OTTHUMP00000064930 antibody
see all
Images
-
Western blot - Anti-SECISBP2 antibody (ab53534)This image is courtesy of an anonymous AbreviewAll lanes : Anti-SECISBP2 antibody (ab53534) at 1/5000 dilution
Lanes 1-2 : Mouse testis lysate - cytosolic fraction (50µg)
Lane 3 : Mouse liver lysate - cytosolic fraction (50µg)
Lane 4 : Mouse kidney lysate - cytosolic fraction (50µg)
Lanes 5-6 : Mouse brain lysate - cytosolic fraction (50µg)
Lane 7 : Human HepG2 Lysate - cytosolic fraction (50µg)
Lane 8 : Rat testis lysate - cytosolic fraction (50µg)
Secondary
All lanes : Donkey anti-goat conjugated to HRP
Predicted band size: 95 kDa
Bar indicates SECISBP2. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SECISBP2 antibody (ab53534)ab53534 at 3ug/ml staining SECISBP2 in human testis tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). The tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9). The HRP-staining procedure was used for detection.
Protocols
Datasheets and documents
References
ab53534 has not yet been referenced specifically in any publications.
