Western blot abreview for Anti-SECISBP2 antibody

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - other (cytosolic fraction diff. tissues: mouse, rat, huma)
Loading amount
50 µg
Specification
cytosolic fraction diff. tissues: mouse, rat, huma
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C

Other product details

Dilution
1/5000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 95 kDa Non-specific: 55, 160 kDa
Positive control
HepG2 cytosol, lane 7
Negative control
mouse testis, lanes 1-2
mouse liver, lane 3
mouse kidney, lane 4
mouse brain, lanes 5-6
rat testis, lane 8

Additional data

Additional Notes
This antibody works well for human, but not mouse and rat. The epitope used to produce the antibody was human, so this is o.k. Since more than the expected 95kD band was observed, IHC will probably not work.

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Submitted Sep 10 2007

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