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Alexa Fluor® 488-conjugated antibodies

Related

  • Alexa Fluor® antibodies
    • Alexa Fluor® 488 conjugated secondaries
      • Alexa Fluor® 594 conjugated secondaries
        • Alexa Fluor® 647 conjugated secondaries
          • Alexa Fluor® 488 conjugated primary antibodies
              • Cell imaging
                • Direct vs indirect immunofluorescence
                  • Fluorochrome chart
                    • ICC protocol
                      • Advantages of Alexa Fluor® secondaries
                        • Tips for ICC experiments
                            • Western blot
                              • See how chemiluminescence compares to fluorescence WB

                                Alexa Fluor® 488 conjugated antibodies advantages compared to Fluorescein isothiocyanate (FITC) make it the most used green fluorophore for fluorescent cell staining.

                                Comparable spectral properties and quantum yield.

                                Brighter and more photostable when conjugated to antibodies. Dapi

                                Use Alexa Fluor® 488 conjugated to secondary antibodies to get bright and sensitive immunofluorescent staining in cell and tissue fluorescent microscopy. The absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell staining  of multiple proteins with Alexa Fluor® 594 and Alexa Fluor® 647 conjugated secondary antibodies.


                                FITC

                                Alexa Fluor® 488

                                Benefits

                                Spectrum

                                Absorption max.: 495 nm

                                Emission max.:

                                519 nm

                                Absorption max.: 495 nm

                                Emission max.:

                                519 nm

                                Comparable spectra means you don’t need to change any settings or filters in your equipment.

                                Photostability

                                ♦ ♦

                                ♦ ♦ ♦ ♦ ♦

                                Alexa Fluor® 488 high photostability allows more time for observation and image capture.

                                Brightness

                                ♦ ♦ ♦

                                ♦ ♦ ♦ ♦ ♦

                                Detect low-abundance proteins with great sensitivity due to Alexa Fluor® 488 low self-quenching and high fluorescence quantum yield.

                                ​​

                                A. Annexin V labeled with Alexa Fluor® 488 in frozen rat placenta section by IHC (immunohistochemistry). Green - Anti-Annexin V antibody [EPR3980] (Alexa Fluor® 488) (ab201540); Red - Anti-Tubulin antibody [YOL1/34] (Alexa Fluor® 647) (ab195884); Blue - DAPI.

                                B. Lamin B1 labeled with Alexa Fluor® 488 in HUVEC cells using Alexa Fluor® 488-conjugated secondary antibody.  Green - Anti-Lamin B1 antibody (ab16048), secondary antibody Goat Anti-Rabbit  IgG H&L (Alexa Fluor® 488) (ab150077); Red - Anti-alpha  Tubulin antibody (ab7291), secondary antibody  Goat anti-mouse  IgG H&L (Alexa Fluor® 647) (ab150115);  Blue - DAPI.

                                Quick guide to using Alexa Fluor® 488
                                ​

                                Excitation laser

                                Argon ion laser (488 nm)

                                Emission filter

                                Confocal microscopy: FITC or Alexa Fluor® 488

                                Flow cytometry: Band pass (BP) filter 525/30

                                Compatible ​fluorophores for multiple staining 

                                DAPI, Alexa Fluor® 594, and Alexa Fluor® 647


                                ​Choosing an Alexa Fluor® 488 secondary antibody

                                Getting the right secondary antibody to your primary antibody is essential for sensitive protein detection. Make sure you get bright and sensitive staining in immunofluorescence, flow cytometry and immunohistochemistry by choosing one of our Alexa Fluor® 488 conjugated secondary antibodies.

                                Primary antibody raised in

                                Use secondary antibody

                                Rabbit

                                Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

                                Donkey Anti-Rabbit IgG H&L (Alexa Fluor® ​488) (ab150073)

                                Mouse

                                Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

                                Goat

                                Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150129)

                                Rat

                                Rabbit Anti-Rat ​IgG H&L (Alexa Fluor® 488) (ab150157)

                                • Find other Alexa Fluor® secondary antibodies

                                To minimize background due to cross-species reactivity in multiple staining, we recommend the use of Alexa Fluor® pre-adsorbed secondary antibodies. Pre-adsorbed secondary antibodies have gone through an extra purification step to minimize non-specific binding.

                                • More on Pre-adsorbed antibodies

                                Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

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