Alexa Fluor® 488-conjugated antibodies
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Alexa Fluor® 488 conjugated antibodies advantages compared to Fluorescein isothiocyanate (FITC) make it the most used green fluorophore for fluorescent cell staining.
Comparable spectral properties and quantum yield.
Brighter and more photostable when conjugated to antibodies. Dapi
Use Alexa Fluor® 488 conjugated to secondary antibodies to get bright and sensitive immunofluorescent staining in cell and tissue fluorescent microscopy. The absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell staining of multiple proteins with Alexa Fluor® 594 and Alexa Fluor® 647 conjugated secondary antibodies.
FITC | Alexa Fluor® 488 | Benefits | |
|---|---|---|---|
Spectrum | Absorption max.: 495 nm Emission max.: 519 nm | Absorption max.: 495 nm Emission max.: 519 nm | Comparable spectra means you don’t need to change any settings or filters in your equipment. |
Photostability | ♦ ♦ | ♦ ♦ ♦ ♦ ♦ | Alexa Fluor® 488 high photostability allows more time for observation and image capture. |
Brightness | ♦ ♦ ♦ | ♦ ♦ ♦ ♦ ♦ | Detect low-abundance proteins with great sensitivity due to Alexa Fluor® 488 low self-quenching and high fluorescence quantum yield. |
A. Annexin V labeled with Alexa Fluor® 488 in frozen rat placenta section by IHC (immunohistochemistry). Green - Anti-Annexin V antibody [EPR3980] (Alexa Fluor® 488) (ab201540); Red - Anti-Tubulin antibody [YOL1/34] (Alexa Fluor® 647) (ab195884); Blue - DAPI.
B. Lamin B1 labeled with Alexa Fluor® 488 in HUVEC cells using Alexa Fluor® 488-conjugated secondary antibody. Green - Anti-Lamin B1 antibody (ab16048), secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077); Red - Anti-alpha Tubulin antibody (ab7291), secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 647) (ab150115); Blue - DAPI.
Quick guide to using Alexa Fluor® 488
Excitation laser | Argon ion laser (488 nm) |
Emission filter | Confocal microscopy: FITC or Alexa Fluor® 488 Flow cytometry: Band pass (BP) filter 525/30 |
Compatible fluorophores for multiple staining |
Choosing an Alexa Fluor® 488 secondary antibody
Getting the right secondary antibody to your primary antibody is essential for sensitive protein detection. Make sure you get bright and sensitive staining in immunofluorescence, flow cytometry and immunohistochemistry by choosing one of our Alexa Fluor® 488 conjugated secondary antibodies.
Primary antibody raised in | Use secondary antibody |
|---|---|
Rabbit | |
Mouse | |
Goat | |
Rat |
To minimize background due to cross-species reactivity in multiple staining, we recommend the use of Alexa Fluor® pre-adsorbed secondary antibodies. Pre-adsorbed secondary antibodies have gone through an extra purification step to minimize non-specific binding.