Alexa Fluor® 488-conjugated antibodies

Alexa Fluor® 488 conjugated antibodies advantages compared to Fluorescein isothiocyanate (FITC) make it the most used green fluorophore for fluorescent cell staining.

Comparable spectral properties and quantum yield.

Brighter and more photostable when conjugated to antibodies. Dapi

Use Alexa Fluor® 488 conjugated to secondary antibodies to get bright and sensitive immunofluorescent staining in cell and tissue fluorescent microscopy. The absorption at 495 nm and emission maximum at 519 nm spectrum make Alexa Fluor 488 secondary antibodies ideal to do fluorescent cell staining  of multiple proteins with Alexa Fluor® 594 and Alexa Fluor® 647 conjugated secondary antibodies.


Alexa Fluor® 488



Absorption max.: 495 nm

Emission max.:

519 nm

Absorption max.: 495 nm

Emission max.:

519 nm

Comparable spectra means you don’t need to change any settings or filters in your equipment.


♦ ♦

♦ ♦ ♦ ♦ ♦

Alexa Fluor® 488 high photostability allows more time for observation and image capture.


♦ ♦ ♦

♦ ♦ ♦ ♦ ♦

Detect low-abundance proteins with great sensitivity due to Alexa Fluor® 488 low self-quenching and high fluorescence quantum yield.


A. Annexin V labeled with Alexa Fluor® 488 in frozen rat placenta section by IHC (immunohistochemistry). Green - Anti-Annexin V antibody [EPR3980] (Alexa Fluor® 488) (ab201540); Red - Anti-Tubulin antibody [YOL1/34] (Alexa Fluor® 647) (ab195884); Blue - DAPI.

B. Lamin B1 labeled with Alexa Fluor® 488 in HUVEC cells using Alexa Fluor® 488-conjugated secondary antibody.  Green - Anti-Lamin B1 antibody (ab16048), secondary antibody Goat Anti-Rabbit  IgG H&L (Alexa Fluor® 488) (ab150077); Red - Anti-alpha  Tubulin antibody (ab7291), secondary antibody  Goat anti-mouse  IgG H&L (Alexa Fluor® 647) (ab150115);  Blue - DAPI.

Quick guide to using Alexa Fluor® 488

Excitation laser

Argon ion laser (488 nm)

Emission filter

Confocal microscopy: FITC or Alexa Fluor® 488

Flow cytometry: Band pass (BP) filter 525/30

Compatible ​fluorophores for multiple staining 

DAPI, Alexa Fluor® 594, and Alexa Fluor® 647

​Choosing an Alexa Fluor® 488 secondary antibody

Getting the right secondary antibody to your primary antibody is essential for sensitive protein detection. Make sure you get bright and sensitive staining in immunofluorescence, flow cytometry and immunohistochemistry by choosing one of our Alexa Fluor® 488 conjugated secondary antibodies.

Primary antibody raised in

Use secondary antibody


Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® ​488) (ab150073)


Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)


Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150129)


Rabbit Anti-Rat ​IgG H&L (Alexa Fluor® 488) (ab150157)

To minimize background due to cross-species reactivity in multiple staining, we recommend the use of Alexa Fluor® pre-adsorbed secondary antibodies. Pre-adsorbed secondary antibodies have gone through an extra purification step to minimize non-specific binding.

Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

Sign up