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Alexa Fluor® conjugated secondary antibodies

Related

  • Alexa Fluor® secondary antibodies
    • Alexa Fluor® 488 conjugated secondaries
      • Alexa Fluor® 594 conjugated secondaries
        • Alexa Fluor® 647 conjugated secondaries
          • Alexa Fluor® primary antibodies
              • Cell imaging
                • Direct vs indirect IF
                  • Fluorochrome chart
                    • Compare Alexa Fluor®488 to FITC
                      • Secondary antibody selection guide
                        • Subcellular and cell membrane markers
                          • Western blot tools
                            • Infrared fluorescence vs chemiluminescence

                                Alexa Fluor® dyes are extensively tested for fluorescent cell staining to ensure bright staining and low background.

                                Customer review

                                Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

                                "It worked perfectly... The intensity of the fluorescence was really great too, strongly recommend." 

                                Features


                                Brightest dyesThe fluorescence intensity of Alexa Fluor® dyes outperform other spectrally similar dyes
                                Greater photostabilityIncreased photostability allows for longer periods of image capture
                                pH insensitivityAlexa Fluor® fluorescence intensity remains high over a broad pH range
                                Good water solubilityGood water solubility prevents the precipitation and aggregation of Alexa Fluor® conjugated antibodies

                                Performance

                                • In house manufacturing within our laboratories guarantees consistency and quality.
                                • Alexa Fluor® conjugates ensure you make the most of your money by providing lower optimal working dilutions.
                                • The wavelength at which Alexa Fluor® conjugates absorb matches the most common operational wavelengths of excitation sources.

                                Conjugation

                                • We currently offer nine Alexa Fluor® dyes covering the whole of the spectrum from the UV to the far infrared.
                                • The Alexa Fluor® dyes have been conjugated to a large number of secondary antibodies raised in different species including donkey, goat and rabbit.
                                • A large number within the range is pre-adsorbed ensuring low species cross-reactivity - thus ideal for multi-color analysis.

                                Validation

                                • Our Alexa Fluor® conjugated secondary antibodies have been tested in a wide range of applications including immunofluorescence/immunocytochemistry (IF/ICC), flow-cytometry, and fluorescent western blot.

                                Alexa Fluor dyes emission and absorption spectra

                                Alexa Fluor®Absorption max (nm)Emission max (nm)

                                Extinction
                                coefficient

                                Quantum yield
                                405402421      35,000         -
                                48849551973,0000.92
                                555555565155,0000.1
                                56857860388,0000.69
                                59459061792,0000.66
                                647650668270,0000.33
                                680679702184,0000.36
                                750749775290,0000.12

                                We now have over 200 different Alexa Fluor® secondary antibody conjugates.

                                Find the right Alexa Fluor® secondary antibody

                                Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

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