Alexa Fluor® conjugated secondary antibodies
Related
Alexa Fluor® dyes are extensively tested for fluorescent cell staining to ensure bright staining and low background.
Customer review
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
"It worked perfectly... The intensity of the fluorescence was really great too, strongly recommend."
Features | |
| Brightest dyes | The fluorescence intensity of Alexa Fluor® dyes outperform other spectrally similar dyes |
| Greater photostability | Increased photostability allows for longer periods of image capture |
| pH insensitivity | Alexa Fluor® fluorescence intensity remains high over a broad pH range |
| Good water solubility | Good water solubility prevents the precipitation and aggregation of Alexa Fluor® conjugated antibodies |
Performance
- In house manufacturing within our laboratories guarantees consistency and quality.
- Alexa Fluor® conjugates ensure you make the most of your money by providing lower optimal working dilutions.
- The wavelength at which Alexa Fluor® conjugates absorb matches the most common operational wavelengths of excitation sources.
Conjugation
- We currently offer nine Alexa Fluor® dyes covering the whole of the spectrum from the UV to the far infrared.
- The Alexa Fluor® dyes have been conjugated to a large number of secondary antibodies raised in different species including donkey, goat and rabbit.
- A large number within the range is pre-adsorbed ensuring low species cross-reactivity - thus ideal for multi-color analysis.
Validation
- Our Alexa Fluor® conjugated secondary antibodies have been tested in a wide range of applications including immunofluorescence/immunocytochemistry (IF/ICC), flow-cytometry, and fluorescent western blot.
Alexa Fluor dyes emission and absorption spectra
| Alexa Fluor® | Absorption max (nm) | Emission max (nm) | Extinction | Quantum yield |
| 405 | 402 | 421 | 35,000 | - |
| 488 | 495 | 519 | 73,000 | 0.92 |
| 555 | 555 | 565 | 155,000 | 0.1 |
| 568 | 578 | 603 | 88,000 | 0.69 |
| 594 | 590 | 617 | 92,000 | 0.66 |
| 647 | 650 | 668 | 270,000 | 0.33 |
| 680 | 679 | 702 | 184,000 | 0.36 |
| 750 | 749 | 775 | 290,000 | 0.12 |
We now have over 200 different Alexa Fluor® secondary antibody conjugates.