Alexa Fluor® conjugated secondary antibodies

Alexa Fluor® dyes are extensively tested for fluorescent cell staining to ensure bright staining and low background.

Customer review

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

"It worked perfectly... The intensity of the fluorescence was really great too, strongly recommend." 

Features


Brightest dyesThe fluorescence intensity of Alexa Fluor® dyes outperform other spectrally similar dyes
Greater photostabilityIncreased photostability allows for longer periods of image capture
pH insensitivityAlexa Fluor® fluorescence intensity remains high over a broad pH range
Good water solubilityGood water solubility prevents the precipitation and aggregation of Alexa Fluor® conjugated antibodies

Performance

  • In-house manufacturing within our laboratories guarantees consistency and quality.
  • Alexa Fluor® conjugates ensure you make the most of your money by providing lower optimal working dilutions.
  • The wavelength at which Alexa Fluor® conjugates absorb matches the most common operational wavelengths of excitation sources.

Conjugation

  • We currently offer nine Alexa Fluor® dyes covering the whole of the spectrum from the UV to the far-infrared.
  • The Alexa Fluor® dyes have been conjugated to a large number of secondary antibodies raised in different species including donkey, goat, and rabbit.
  • A large number within the range is pre-adsorbed ensuring low species cross-reactivity - thus ideal for multi-color analysis.

Validation

  • Our Alexa Fluor® conjugated secondary antibodies have been tested in a wide range of applications including immunofluorescence/immunocytochemistry (IF/ICC), flow-cytometry, and fluorescent western blot.

Alexa Fluor dyes emission and absorption spectra

Alexa Fluor®Absorption max (nm)Emission max (nm)Quantum yield
405402421         -
4884955190.92
5555555650.1
5685786030.69
5945906170.66
6476506680.33
6806797020.36
7507497750.12

We now have over 200 different Alexa Fluor® secondary antibody conjugates.

Find the right Alexa Fluor® secondary antibody

Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.