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Alexa Fluor® dyes are extensively tested for fluorescent cell staining to ensure bright staining and low background.
Customer review
Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
"It worked perfectly... The intensity of the fluorescence was really great too, strongly recommend."
Features | |
Brightest dyes | The fluorescence intensity of Alexa Fluor® dyes outperform other spectrally similar dyes |
Greater photostability | Increased photostability allows for longer periods of image capture |
pH insensitivity | Alexa Fluor® fluorescence intensity remains high over a broad pH range |
Good water solubility | Good water solubility prevents the precipitation and aggregation of Alexa Fluor® conjugated antibodies |
Conjugation
Validation
Alexa Fluor® | Absorption max (nm) | Emission max (nm) | Extinction | Quantum yield |
405 | 402 | 421 | 35,000 | - |
488 | 495 | 519 | 73,000 | 0.92 |
555 | 555 | 565 | 155,000 | 0.1 |
568 | 578 | 603 | 88,000 | 0.69 |
594 | 590 | 617 | 92,000 | 0.66 |
647 | 650 | 668 | 270,000 | 0.33 |
680 | 679 | 702 | 184,000 | 0.36 |
750 | 749 | 775 | 290,000 | 0.12 |
We now have over 200 different Alexa Fluor® secondary antibody conjugates.