For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Take a look at our BETA site and see what we’ve done so far.
Search and browse selected products
Purchase these through your usual distributor
Enjoy the flexibility and freedom of designing a multicolor experiment with a quick and easy guide that helps you select the most appropriate fluorochrome.
Our fluorochrome chart includes
Fluorescent dyes (or fluorochromes) are commonly used as detection reagents in various applications such as cellular imaging and flow cytometry. Fluorochromes absorb light energy of a specific wavelength and re-emit it at a longer wavelength. The wavelengths at which the fluorochrome absorbs and emits light are known as the fluorochrome's excitation and emission spectra, respectively (or just fluorescence spectra).
Instruments requiring the use of fluorescent dyes, such as fluorescence microscopes and flow cytomeres, are equipped with lasers producing light at a particular wavelength so they can excite fluorochromes capable of absorbing light at that specific wavelength. The light emitted by the fluorochrome is then filtered so that each sensor will detect fluorescence only within the filter's range. This fluorescence is the read-out signal provided by the instrument.
Many different fluorochromes have been developed, each one with a particular emission and excitation fluorescence spectra. You can find commercially available fluorescent dyes that absorb and emit light at specific wavelengths across the whole visible spectrum, including the infrared region. Fluorochromes differ in the intensity at which they emit light. Therefore, the fluorochrome brightness will depend on its ability to absorb light and the efficiency at which the absorbed light is converted into emitted light.
Selecting the right fluorochrome may seem difficult if you don’t have adequate tools – particularly when designing a multiplex panel that requires two or more fluorochromes. If this is the case, consider the spectral overlap between fluorochromes and other factors to minimize potential problems, such as fluorescence spillover.
When it comes to selecting the right target and label, we offer the most comprehensive range of highly consistent recombinant conjugated antibodies on the market – this gives you the ultimate flexibility when designing your multiplex experiment. With multiple ways for you to identify the perfect combination of target and label quickly and easily, you have the freedom to design your experiment exactly how you want it.
Our fluorochrome chart explores the most common labels to help you identify the ideal fluorochrome for your multiplex experiment, including a step-by-step guide on how to use the chart to select a suitable fluorochrome.
Ready to start designing your experiment? Download the fluorochrome chart here.