Undissolved particles within buffers (milk powder in blocking buffer for example) potentially can settle on the membrane and create fluorescent artifacts. Therefore, we suggest using high quality reagents, allowing suitable time for all components to fully dissolve, and filter sterilize all buffers.
Handle the membrane with care, use blunt forceps and avoid scratching/creasing to prevent fluorescent artifacts.
Ink fluoresces, so mark the membrane with a pencil rather than pen.
Bromophenol blue itself fluoresces - so either run the dye front off the gel, or cut the gel part off, which contains the dye, prior to transfer.
Protect membrane from light during incubation and wash steps with aluminium foil.